Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Aug;119(4):405-13.
doi: 10.1007/s00412-010-0265-x. Epub 2010 Mar 31.

Aurora B kinase cooperates with CENP-E to promote timely anaphase onset

André F Maia  1 , Tália Feijão, Martijn J M Vromans, Claudio E Sunkel, Susanne M A Lens
Affiliations

Aurora B kinase cooperates with CENP-E to promote timely anaphase onset

André F Maia et al. Chromosoma. 2010 Aug.

Abstract

Error-free chromosome segregation requires that all chromosomes biorient on the mitotic spindle. The motor protein Centromere-associated protein E (CENP-E) facilitates chromosome congression by mediating the lateral sliding of sister chromatids along existing K-fibers, while the mitotic kinase Aurora B detaches kinetochore-microtubule interactions that are not bioriented. Whether these activities cooperate to promote efficient chromosome biorientation and timely anaphase onset is not known. We here show that the chromosomes that fail to congress after CENP-E depletion displayed high centromeric Aurora B kinase activity. This activity destabilized spindle pole proximal kinetochore-microtubule interactions resulting in a checkpoint-dependent mitotic delay that allowed CENP-E-independent chromosome congression, thus reducing chromosome segregation errors. This shows that Aurora B keeps the mitotic checkpoint active by destabilizing kinetochore fibers of polar chromosomes to permit chromosome congression in CENP-E-compromised cells and implies that this kinase normally prevents pole proximal syntelic attachments to allow CENP-E-mediated congression of mono-oriented chromosomes.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Cell Biol. 1997 Dec 15;139(6):1373-82 - PubMed
    1. Nature. 1992 Oct 8;359(6395):536-9 - PubMed
    1. Science. 2006 Jan 20;311(5759):388-91 - PubMed
    1. Mol Biol Cell. 2001 Sep;12(9):2776-89 - PubMed
    1. Curr Biol. 2006 Jun 20;16(12):1194-200 - PubMed

Publication types

LinkOut - more resources