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. 1991 Jun;65(6):3219-26.
doi: 10.1128/JVI.65.6.3219-3226.1991.

A domain at the 3' end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs

R G van der Most  1 , P J Bredenbeek, W J Spaan
Affiliations

A domain at the 3' end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs

R G van der Most et al. J Virol. 1991 Jun.

Abstract

Two murine hepatitis virus strain A59 defective interfering (DI) RNAs were generated by undiluted virus passages. The DI RNAs were encapsidated efficiently. The smallest DI particle, DI-a, contained a 5.5-kb RNA consisting of the following three noncontiguous regions from the MHV-A59 genome, which were joined in frame: the 5'-terminal 3.9 kb, a 798-nucleotide fragment from the 3' end of the polymerase gene, and the 3'-terminal 805 nucleotides. A full-length cDNA clone of the DI-a genome was constructed and cloned downstream of the bacteriophage T7 promoter. Transcripts derived from this clone, pMIDI, were used for transfection of MHV-A59-infected cells and found to be amplified and packaged. Deletion analysis of pMIDI allowed us to identify a 650-nucleotide region derived from the 3' end of the second open reading frame of the polymerase gene that was required for efficient encapsidation.

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References

    1. Arch Gesamte Virusforsch. 1974;44(3):298-302 - PubMed
    1. Cell. 1986 Jan 17;44(1):137-45 - PubMed
    1. Virology. 1981 Jan 30;108(2):424-34 - PubMed
    1. Nucleic Acids Res. 1983 Feb 11;11(3):883-91 - PubMed
    1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed

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