Functional analyses of the domain structure of microtubule-associated protein-4 (MAP-U)
- PMID: 2033072
Functional analyses of the domain structure of microtubule-associated protein-4 (MAP-U)
Abstract
Bovine microtubule-associated protein-4 (MAP-4), which was previously named MAP-U, consists of an amino-terminal projection domain (N-domain) and a carboxyl-terminal microtubule-binding domain (C-domain) (Aizawa, H., Emori, Y., Murofushi, H., Kawasaki, H., Sakai, H., and Suzuki, K. (1990) J. Biol. Chem. 265, 13849-13855). The C-domain contains a region rich in proline (Pro-rich region) and a region containing four assembly-promoting sequences (AP sequence region) which is shared by MAP-2 and tau. We purified a series of truncated fragments of MAP-4 expressed in Escherichia coli. An N-domain fragment did not bind to microtubules, while a C-domain fragment promoted microtubule assembly. Both of the fragments corresponding to the Pro-rich region (P fragment) and the AP sequence region (A4 fragment) promoted tubulin polymerization, although the A4 fragment had lower activity than intact MAP-4 and P fragment. A4 fragment produced morphologically normal microtubules whereas P fragment produced abnormal microtubules such as duplex microtubules and tight bundles of microtubules with diverse diameters. We concluded that both Pro-rich and AP sequence regions take part in the promotion of tubulin polymerization, and that the former is important for the MAP to bind to microtubules with high efficiency and the latter is essential for the formation of microtubules with normal morphology.
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