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. 2010 Feb;29(2):127-35.
doi: 10.1007/s10930-010-9232-x.

Phosphoinositide 3-kinase gamma has multiple phospholipid binding sites

Carsten Schmidt  1 , Margret Schilli-Westermann, Reinhard Klinger, Cornelia Kirsch
Affiliations

Phosphoinositide 3-kinase gamma has multiple phospholipid binding sites

Carsten Schmidt et al. Protein J. 2010 Feb.

Abstract

Phosphoinositide 3-kinase gamma is a multifunctional enzyme with lipid and protein kinase activities that also acts as a scaffold protein in many diverse signalling processes. The enzyme contains five different domains, but their individual contributions to membrane binding are not fully understood. Here, using in vitro liposome binding assays of individual domains and deletion constructs of human phosphoinositide 3-kinase gamma, we show that each domain is capable of binding anionic phospholipids to varying degrees, depending on the charge of the anionic substrate. Moreover, with the exception of the C2-domain, deletion of any single protein domain results in a complete loss of kinase activity toward both lipids and proteins.

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References

    1. J Biol Chem. 2000 Feb 4;275(5):3699-705 - PubMed
    1. FEBS Lett. 2002 Oct 30;531(1):28-32 - PubMed
    1. Thromb Haemost. 2006 Jan;95(1):29-35 - PubMed
    1. FEBS Lett. 2001 Feb 9;490(1-2):49-53 - PubMed
    1. Annu Rev Biophys Biomol Struct. 2000;29:49-79 - PubMed

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