Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Apr;11(4):2081-95.
doi: 10.1128/mcb.11.4.2081-2095.1991.

Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells

B E Pearson  1 , H P Nasheuer, T S Wang
Affiliations

Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells

B E Pearson et al. Mol Cell Biol. 1991 Apr.

Abstract

We have investigated the DNA polymerase alpha promoter sequence requirements for the expression of a heterologous gene in actively cycling cells and following serum addition to serum-deprived cells. An 11.4-kb genomic clone that spans the 5' end of this gene and includes 1.62 kb of sequence upstream from the translation start site was isolated. The transcription start site was mapped at 46 +/- 1 nucleotides upstream from the translation start site. The upstream sequence is GC rich and lacks a TATA sequence but has a CCAAT sequence on the opposite strand. Analysis of a set of deletion constructs in transient transfection assays demonstrated that efficient expression of the reporter in cycling cells requires 248 bp of sequence upstream from the cap site. Clustered within these 248 nucleotides are sequences similar to consensus sequences for Sp1-, Ap1-, Ap2-, and E2F-binding sites. The CCAAT sequence and the potential E2F- and Ap1-binding sites are shown to be protected from DNase I digestion by partially purified nuclear proteins. The DNA polymerase alpha promoter can confer upon the reporter an appropriate, late response to serum addition. No single sequence element could be shown to confer serum inducibility. Rather, multiple sequence elements appear to mediate the full serum response.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Cell Biol. 1988 May;8(5):2140-8 - PubMed
    1. Cell. 1990 Jun 29;61(7):1161-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Aug;85(16):5894-8 - PubMed
    1. Cell. 1988 Nov 4;55(3):395-7 - PubMed
    1. Mol Cell Biol. 1988 Nov;8(11):5016-25 - PubMed

Publication types