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Review
. 2009 Dec;11(4):639-52.
doi: 10.1208/s12248-009-9140-1. Epub 2009 Sep 9.

Lipidic systems for in vivo siRNA delivery

Sherry Y Wu  1 , Nigel A J McMillan
Affiliations
Review

Lipidic systems for in vivo siRNA delivery

Sherry Y Wu et al. AAPS J. 2009 Dec.

Abstract

The ability of small-interfering RNA (siRNA) to silence specific target genes not only offers a tool to study gene function but also represents a novel approach for the treatment of various human diseases. Its clinical use, however, has been severely hampered by the lack of delivery of these molecules to target cell populations in vivo due to their instability, inefficient cell entry, and poor pharmacokinetic profile. Various delivery vectors including liposomes, polymers, and nanoparticles have thus been developed in order to circumvent these problems. This review presents a comprehensive overview of the barriers and recent progress for both local and systemic delivery of therapeutic siRNA using lipidic vectors. Different strategies for formulating these siRNA-loaded lipid particles as well as the general concern about their safe use in vivo will also be discussed. Finally, current advances in the targeted delivery of siRNA and their impacts on the field of RNA interference (RNAi)-based therapy will be presented.

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Figures

Fig. 1
Fig. 1
Formulation strategies for preparation of siRNA-loaded PEGylated lipid particles
Fig. 2
Fig. 2
Strategies to enhance the delivery efficiency of PEGylated lipid particles
See this image and copyright information in PMC

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