Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jul;31(7):770-6.
doi: 10.1136/gut.31.7.770.

Effects of an enteric anaerobic bacterial culture supernatant and deoxycholate on intestinal calcium absorption and disaccharidase activity

K Walshe  1 , M J Healy, A B Speekenbrink, C T Keane, D G Weir, R R O'Moore
Affiliations

Effects of an enteric anaerobic bacterial culture supernatant and deoxycholate on intestinal calcium absorption and disaccharidase activity

K Walshe et al. Gut. 1990 Jul.

Abstract

Fifty two strains of anaerobic bacteria isolated from the upper gut of patients with small intestinal bacterial overgrowth were screened for phospholipase activity. Bacteroides melaninogenicus spp intermedius had the greatest activity. The effects of culture supernatants of this organism and deoxycholate on intestinal calcium absorption and disaccharidase activity were studied using a rat closed loop model. The supernatant decreased the in vitro uptake of calcium by 15% (p less than 0.001). Deoxycholate reduced calcium uptake by 16% (p less than 0.001). Combined culture supernatant and deoxycholate reduced calcium uptake by 39% (p less than 0.001) suggesting a potentiation of supernatant activity by deoxycholate. Culture supernatant and deoxycholate, both alone and combined, significantly reduced lactase, sucrase, and maltase activity. Electron microscopic evidence showed degeneration of microvilli, disruption of mitochondrial structure, and swelling of the endoplasmic reticulum after exposure of the intestinal loops to the supernatant or deoxycholate.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Gastroenterology. 1978 Nov;75(5):791-5 - PubMed
    1. J Rheumatol. 1979 Jan-Feb;6(1):57-64 - PubMed
    1. Gastroenterology. 1979 May;76(5 Pt 1):1035-55 - PubMed
    1. Biochim Biophys Acta. 1975 Mar 25;415(1):81-47 - PubMed
    1. Br Med J. 1977 May 21;1(6072):1310-2 - PubMed

LinkOut - more resources