Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1990;3(1):1-27.
doi: 10.1016/0892-0354(90)90011-g.

Virological applications of the grid-cell-culture technique

A D Hyatt  1 , B T Eaton
Affiliations
Review

Virological applications of the grid-cell-culture technique

A D Hyatt et al. Electron Microsc Rev. 1990.

Abstract

Whole mounts of intact virus-infected cells have been used for several decades to examine virus-cell relationships and virus structure. The general concept of studying virus structure in association with the host cell has recently been expanded to reveal interactions between viruses and the cytoskeleton. The procedure permits utilization of immuno-gold protocols using both the transmission and scanning electron microscopes. The grid-cell-culture technique is reviewed to explain how it can be exploited to provide valuable information about virus structure and replication in both diagnostic and research laboratories. The use of the technique at the research level is discussed using bluetongue virus as a model. The procedure can provide basic structural information about intact virions and additional data on the intracellular location of viruses and virus-specific structures and about the mode of virus release from infected cells. Application of immunoelectron microscopy reveals information on the protein composition of not only released virus particles but also cell surface and cytoskeletal-associated viruses and virus-specific structures. Collectively, this simple and physically gentle technique has provided information which would otherwise be difficult to obtain.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Adrian M., Dubochet J., Lepault J., McDowall A.W. Cryo-electron microscopy of viruses. Nature, Lond. 1984;308:32–36. - PubMed
    1. Bauer H., Medzon E.L. Freeze-etching technique for the study of virus ultrastructure. In: Maramorosch K., Kaprowski H., editors. Vol. 5. Academic Press; New York: 1971. pp. 481–495. (Methods in Virology).
    1. Beesley J.E. Bioapplication of colloidal gold in microbiological immunocytochemistry. Scanning Microsc. 1988;2:1055–1068. - PubMed
    1. Bell P.B. The application of scanning electron microscopy to the study of the cytoskeleton of cells in culture. Scanning Electron Microsc. 1981:139–157. Part 2. - PubMed
    1. Bell P.B., Lindroth M., Fredriksson B.A. Preparation of cytoskeletons of cells in culture for high resolution scanning and scanning transmission electron microscopy. Scanning Microsc. 1988;2:1647–1661. - PubMed

MeSH terms

LinkOut - more resources