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. 2009 Sep 4;386(4):715-7.
doi: 10.1016/j.bbrc.2009.06.111. Epub 2009 Jun 25.

Knockout of the regulatory factor X1 gene leads to early embryonic lethality

Chenzhuo Feng  1 , Wenhao Xu, Zhiyi Zuo
Affiliations

Knockout of the regulatory factor X1 gene leads to early embryonic lethality

Chenzhuo Feng et al. Biochem Biophys Res Commun. .

Abstract

The biological function of regulatory factor X1 (RFX1), the prototype member of the transcription factor RFX family, is not clear. We have used gene trap technique to disrupt the expression of RFX1 in mice. Although, heterozygous RFX1(+/-) mice appear normal and fertile, homozygous RFX1(-/-) embryos died at an early stage (most likely before embryonic day 2.5). Our results indicate that RFX1 regulates expression of genes that are essential for early embryonic development/survival and that RFX1 function can not be compensated by other RFX1 family members.

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Figures

Fig. 1
Fig. 1. The gene trap mutation in the mouse RFX1 gene
The pGT2Lxf vector was integrated into the second intron of the RFX1 gene that consists of 21exons. The locations of RFX1-specific and vector-specific primers used in PCR-based genotyping of embryos and mice are shown in the top panel. SA: splice acceptor sequence; PA: polyadenylation signal. A representative gel image of the PCR products is shown in the bottom panel. The wild-type and mutant alleles produce products of 620 and 385 nucleotides, respectively.
See this image and copyright information in PMC

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