doi: 10.1007/978-1-60327-302-2_4.
Construction of phage antibody repertoires from the blood of West Nile virus-infected donors
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- PMID: 19554286
- DOI: 10.1007/978-1-60327-302-2_4
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Construction of phage antibody repertoires from the blood of West Nile virus-infected donors
Methods Mol Biol.
2009.
Abstract
A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (PCR). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies.
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