Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 May;53(3):249-56.
doi: 10.1007/s10384-009-0655-x. Epub 2009 May 31.

Subretinal delivery of immunoglobulin G with gold nanoparticles in the rabbit eye

Atsushi Hayashi  1 , Ayman Naseri, Mark E Pennesi, Eugene de Juan Jr
Affiliations

Subretinal delivery of immunoglobulin G with gold nanoparticles in the rabbit eye

Atsushi Hayashi et al. Jpn J Ophthalmol. 2009 May.

Abstract

Purpose: To examine the feasibility of subretinal delivery of immunoglobulin G (IgG) adsorbed onto gold nanoparticles (GNPs) and its histologic distribution in the rabbit retina after the injection.

Methods: Goat IgG was adsorbed onto GNPs electrostatically. Goat IgG-adsorbed GNPs or buffer with goat IgG was injected into the subretinal space of rabbit eyes and followed up for 3 months by examination of fundus photographs, immunohistochemistry against goat IgG, and transmission electron microscopy (TEM). Human retinal pigment epithelial cells (ARPE-19 cells) were cultured, and cell proliferation with or without GNPs was assayed.

Results: At 1 week after the subretinal injection of goat IgG-adsorbed GNPs, retinal degeneration was observed in the outer retina, and goat IgG was immunolabeled in the retinal pigment epithelium (RPE) and the photoreceptor cells. TEM showed GNPs located in the outer segments and in the lysosomes in the RPE at 1 month and no apparent cytotoxicity of the RPE. There were no inhibitory effects of GNPs on proliferation of ARPE-19 cells.

Conclusions: Goat IgG was successfully delivered into photoreceptor cells and RPE using GNPs, though retinal degeneration in the outer retina occurred in this model. This might be an alternative drug delivery method to photoreceptors and RPE.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Invest Ophthalmol Vis Sci. 1996 Oct;37(11):2146-56 - PubMed
    1. Clin Cancer Res. 2005 May 1;11(9):3530-4 - PubMed
    1. Nano Lett. 2005 May;5(5):829-34 - PubMed
    1. Curr Opin Ophthalmol. 2007 Nov;18(6):502-8 - PubMed
    1. Adv Colloid Interface Sci. 2007 Oct 31;134-135:167-74 - PubMed

MeSH terms

LinkOut - more resources