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. 1991 Oct;229(3):479-82.
doi: 10.1007/BF00267472.

The gltF gene of Klebsiella pneumoniae: cloning and initial characterization

T Kuczius  1 , T Eitinger, R D'Ari, H Castorph, D Kleiner
Affiliations

The gltF gene of Klebsiella pneumoniae: cloning and initial characterization

T Kuczius et al. Mol Gen Genet. 1991 Oct.

Abstract

From a gene bank of Klebsiella pneumoniae M5a1, a 1.7 kb gene fragment was isolated which was able to restore the Ntr+ phenotype and ammonium (methylammonium) transport, but not glutamate synthase in an Escherichia coli glt mutant (glutamate synthase deficiency). The fragment strongly hybridized with the gltF regulatory gene from E. coli. After subcloning the fragment into an overexpression vector, a protein with a molecular weight of 27,000 dalton was identified as the gene product. The results indicate that the fragment cloned contains the gltF gene from K.pneumoniae.

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