Unusual Sp1-GC box interaction in a parvovirus promoter
- PMID: 1942250
- PMCID: PMC250737
- DOI: 10.1128/JVI.65.12.6661-6670.1991
Unusual Sp1-GC box interaction in a parvovirus promoter
Abstract
The P4 promoter of the parvovirus minute virus of mice contains a single degenerate GC box sequence which binds the transcription factor Sp1 with high affinity. The two promoters of murine Sp1 were affinity purified, and their interactions with the P4 promoter were examined. Several unusual features were observed. Methylation interference experiments demonstrated that Sp1 makes contacts with both DNA strands, including the central guanine as well as an adenine residue on the cytidine-rich strand of the GC box. UV photocross-linking revealed that the 95- and the 105-kDa promoters cross-link exclusively to opposite strands of the GC box. These results suggest that the phosphorylation of the 95-kDa Sp1 promoter results in a change in the way Sp1 is positioned on the P4 GC box and identifies a high-affinity GC box motif.
Similar articles
-
A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription.J Virol. 1989 Nov;63(11):4814-23. doi: 10.1128/JVI.63.11.4814-4823.1989. J Virol. 1989. PMID: 2795719 Free PMC article.
-
Binding of transcription factor Sp1 to GC box DNA revealed by footprinting analysis: different contact of three zinc fingers and sequence recognition mode.Biochemistry. 1993 Jun 15;32(23):5994-6001. doi: 10.1021/bi00074a010. Biochemistry. 1993. PMID: 7685185
-
Functional role of a conformationally flexible homopurine/homopyrimidine domain of the androgen receptor gene promoter interacting with Sp1 and a pyrimidine single strand DNA-binding protein.Mol Endocrinol. 1997 Jan;11(1):3-15. doi: 10.1210/mend.11.1.9868. Mol Endocrinol. 1997. PMID: 8994183
-
Regulation of transcription from the hepatitis B virus major surface antigen promoter by the Sp1 transcription factor.J Virol. 1992 Dec;66(12):6912-21. doi: 10.1128/JVI.66.12.6912-6921.1992. J Virol. 1992. PMID: 1331502 Free PMC article.
-
The promoter activity of long terminal repeats of the HERV-H family of human retrovirus-like elements is critically dependent on Sp1 family proteins interacting with a GC/GT box located immediately 3' to the TATA box.J Virol. 1996 Jan;70(1):188-98. doi: 10.1128/JVI.70.1.188-198.1996. J Virol. 1996. PMID: 8523525 Free PMC article.
Cited by
-
cis-acting sequences in the Aleutian mink disease parvovirus late promoter important for transcription: comparison to the canine parvovirus and minute virus of mice.J Virol. 1993 Apr;67(4):1887-95. doi: 10.1128/JVI.67.4.1887-1895.1993. J Virol. 1993. PMID: 8383216 Free PMC article.
-
The adeno-associated virus type 2 p40 promoter requires a proximal Sp1 interaction and a p19 CArG-like element to facilitate Rep transactivation.J Virol. 1997 Jun;71(6):4300-9. doi: 10.1128/JVI.71.6.4300-4309.1997. J Virol. 1997. PMID: 9151818 Free PMC article.
-
ras oncogene-dependent activation of the P4 promoter of minute virus of mice through a proximal P4 element interacting with the Ets family of transcription factors.J Virol. 1996 Mar;70(3):1331-9. doi: 10.1128/JVI.70.3.1331-1339.1996. J Virol. 1996. PMID: 8627649 Free PMC article.
-
The GC box and TATA transcription control elements in the P38 promoter of the minute virus of mice are necessary and sufficient for transactivation by the nonstructural protein NS1.J Virol. 1992 Jun;66(6):3776-83. doi: 10.1128/JVI.66.6.3776-3783.1992. J Virol. 1992. PMID: 1583730 Free PMC article.
-
Transcriptional activation by the parvoviral nonstructural protein NS-1 is mediated via a direct interaction with Sp1.Mol Cell Biol. 1995 Jan;15(1):524-33. doi: 10.1128/MCB.15.1.524. Mol Cell Biol. 1995. PMID: 7799962 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
