Nucleases and helicases take center stage in homologous recombination
- PMID: 19375328
- DOI: 10.1016/j.tibs.2009.01.010
Nucleases and helicases take center stage in homologous recombination
Abstract
Homologous recombination (HR)-mediated DNA double-strand break repair maintains genome integrity. Although long-studied, an understanding of two essential steps in this process -- the resection of DNA ends to produce recombinogenic 3' single-stranded DNA tails and the resolution of recombination intermediates -- has remained elusive. Recent findings show an unexpected role for the Sgs1 (BLM) helicase and Dna2 nuclease in end resection, and provide mechanistic insight into the initiation of 5'-3' resection as well as its regulation by the cell cycle and the DNA damage response. Moreover, the identification of a novel Holliday junction resolvase, Yen1 (GEN1), and several helicases that dismantle strand invasion intermediates has increased the repertoire of nucleases and helicases capable of resolving recombination intermediates.
Similar articles
-
The Caenorhabditis elegans homolog of Gen1/Yen1 resolvases links DNA damage signaling to DNA double-strand break repair.PLoS Genet. 2010 Jul 15;6(7):e1001025. doi: 10.1371/journal.pgen.1001025. PLoS Genet. 2010. PMID: 20661466 Free PMC article.
-
Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1.Nat Commun. 2016 Oct 25;7:13157. doi: 10.1038/ncomms13157. Nat Commun. 2016. PMID: 27779184 Free PMC article.
-
Identification of Holliday junction resolvases from humans and yeast.Nature. 2008 Nov 20;456(7220):357-61. doi: 10.1038/nature07470. Nature. 2008. PMID: 19020614
-
GEN1/Yen1 and the SLX4 complex: Solutions to the problem of Holliday junction resolution.Genes Dev. 2010 Mar 15;24(6):521-36. doi: 10.1101/gad.1903510. Epub 2010 Mar 4. Genes Dev. 2010. PMID: 20203129 Free PMC article. Review.
-
Holliday junction processing enzymes as guardians of genome stability.Trends Biochem Sci. 2014 Sep;39(9):409-19. doi: 10.1016/j.tibs.2014.07.003. Epub 2014 Aug 14. Trends Biochem Sci. 2014. PMID: 25131815 Review.
Cited by
-
Coincident resection at both ends of random, γ-induced double-strand breaks requires MRX (MRN), Sae2 (Ctp1), and Mre11-nuclease.PLoS Genet. 2013 Mar;9(3):e1003420. doi: 10.1371/journal.pgen.1003420. Epub 2013 Mar 28. PLoS Genet. 2013. PMID: 23555316 Free PMC article.
-
Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination.J Biol Chem. 2012 Aug 17;287(34):28727-37. doi: 10.1074/jbc.M112.373290. Epub 2012 Jun 29. J Biol Chem. 2012. PMID: 22761450 Free PMC article.
-
Tissue specificity of DNA damage response and tumorigenesis.Cancer Biol Med. 2019 Aug;16(3):396-414. doi: 10.20892/j.issn.2095-3941.2019.0097. Cancer Biol Med. 2019. PMID: 31565474 Free PMC article.
-
High RAD51 gene expression is associated with aggressive biology and with poor survival in breast cancer.Breast Cancer Res Treat. 2022 May;193(1):49-63. doi: 10.1007/s10549-022-06552-0. Epub 2022 Mar 6. Breast Cancer Res Treat. 2022. PMID: 35249172 Free PMC article.
-
RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif.J Biol Chem. 2011 Oct 28;286(43):37328-34. doi: 10.1074/jbc.M111.290015. Epub 2011 Sep 8. J Biol Chem. 2011. PMID: 21903585 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
