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. 2009 Jun 3;28(11):1589-600.
doi: 10.1038/emboj.2009.89. Epub 2009 Apr 9.

SLP-2 is required for stress-induced mitochondrial hyperfusion

Daniel Tondera  1 , Stéphanie Grandemange, Alexis Jourdain, Mariusz Karbowski, Yves Mattenberger, Sébastien Herzig, Sandrine Da Cruz, Pascaline Clerc, Ines Raschke, Carsten Merkwirth, Sarah Ehses, Frank Krause, David C Chan, Christiane Alexander, Christoph Bauer, Richard Youle, Thomas Langer, Jean-Claude Martinou
Affiliations

SLP-2 is required for stress-induced mitochondrial hyperfusion

Daniel Tondera et al. EMBO J. .

Abstract

Mitochondria are dynamic organelles, the morphology of which results from an equilibrium between two opposing processes, fusion and fission. Mitochondrial fusion relies on dynamin-related GTPases, the mitofusins (MFN1 and 2) in the outer mitochondrial membrane and OPA1 (optic atrophy 1) in the inner mitochondrial membrane. Apart from a role in the maintenance of mitochondrial DNA, little is known about the physiological role of mitochondrial fusion. Here we report that mitochondria hyperfuse and form a highly interconnected network in cells exposed to selective stresses. This process precedes mitochondrial fission when it is triggered by apoptotic stimuli such as UV irradiation or actinomycin D. Stress-induced mitochondrial hyperfusion (SIMH) is independent of MFN2, BAX/BAK, and prohibitins, but requires L-OPA1, MFN1, and the mitochondrial inner membrane protein SLP-2. In the absence of SLP-2, L-OPA1 is lost and SIMH is prevented. SIMH is accompanied by increased mitochondrial ATP production and represents a novel adaptive pro-survival response against stress.

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Figures

Figure 1
Figure 1
Mitochondrial tubulation in response to stress stimuli requires Opa1 and Mfn1. (A, B) Wild-type MEFs after exposure to 60 mJ/cm2 UV-C, 3 μg/ml Act D or 10 μM CHX. (A) MEFs stained with antibodies to cytochrome c were analyzed in untreated conditions and 9 h after stress exposure by fluorescence microscopy. Scale bar, 25 μm. (B) Quantification of cells with a majority of connected, very long (>5 μm) tubular mitochondria the extremities of which are difficult to visualize (defined as hyperfused mitochondria) at the indicated time points after stress exposure. Data represent the mean±s.d. of three independent experiments, each with >300 cells counted per condition. (C, D) Wild type, Mfn1−/−, Mfn2−/−, Mfn1/2−/−, Opa1−/−, and Bax/Bak−/− MEFs after exposure to UV-C irradiation. (C) Quantification of cells with hyperfused mitochondria 9 h after exposure with 60 mJ/cm2 UV-C, 3 μg/ml Act D, or 10 μM CHX. Data represent the mean±s.d. of three independent experiments, each with >300 cells counted per condition. Wild-type MEFs shown here are controls for Mfn1−/− cells and are representative of all WT MEFs used as controls for mutant MEFs. (D) MEFs stained with antibodies to cytochrome c were analysed in untreated conditions and 9 h after UV-C irradiation by fluorescence microscopy. Scale bar, 25 μm.
Figure 2
Figure 2
SIMH is a result of increased mitochondrial fusion activity. (A) Visualization and (B) quantification of mitochondrial fusion using mito-PAGFP in DMSO-treated Mfn2−/− MEFs (UT cells) (n=27), as well as Mfn2−/− MEFs 3 h (n=30) and 7 h (n=30) after CHX treatment. Regions of interest within mito-PAGFP-expressing cells were photoactivated using 405-nm light, followed by whole-cell imaging with 488-nm light. Pre-activation images were taken with higher detector gain, and after the activation the gain was adjusted to avoid oversaturation of activated mitochondria. Post-acquisition processing was performed using MetaMorph software. The data represent mean±s.e.m. P-values (versus untreated Mfn2−/−), t-test, two-tailed, unpaired. (C) Morphology of mitochondria in wild-type, Mfn1−/−, Mfn2−/−, Mfn1/2−/−, and Opa1−/− MEFs transiently expressing the dominant-negative DRP1 mutant HA-DRP1-K38. Mitochondria were stained using MitoTracker and Drp1 K38A revealed using an antibody to HA tag. (D) Quantification of cells described in (C) with elongated mitochondrial morphology. As a control, cells were transfected with GFP. Data represent the mean±s.d. of three independent experiments, each with >300 cells counted per condition. (E) Subcellular fractionation of untreated WT MEFs and after exposure to 60 mJ/cm2 UV-C, 3 μg/ml ActD or 10 μM CHX. Cells were harvested at the indicated time points and mitochondria were isolated. Cytoplasmic and mitochondrial fractions were analysed by SDS–PAGE using antibodies against DRP1 and FIS1, respectively. Actin served as a loading control for the cytoplasmic fraction. The data are representative of three independent experiments. **P<0.01; ***P<0.005; students t-test, unpaired, two-tailed.
Figure 3
Figure 3
Stress-induced mitochondrial hyperfusion requires SLP-2. (A) Quantification of mitochondrial morphology in wild-type MEFs stably expressing control shRNA against Luciferase (shLuc) or shRNA against SLP-2 (shSLP-2) 8 h after exposure to 60 mJ/cm2 UV-C or 10 μM CHX. Tubular mitochondria are defined as isolated mitochondria with visible free ends; hyperfused mitochondria form a mesh of interconnected organelles, the extremities of which were difficult to visualize. (B) Immunoblot analysis of cells described in (A) at the indicated time points after CHX treatment. Whole-cell lysates were analysed by SDS–PAGE using antibodies against OPA1 and SLP-2. (C) Quantification of mitochondrial morphology in wild-type MEFs stably expressing shSLP-2 after transient transfection of FLAG-tagged rat OPA1 variant 1 (rOPA1-V1) and its non-cleavable deletion mutant (rOPA1-V1-ΔS1). Cells exposed to 10 μM CHX for 8 h were stained with MitoTracker and antibodies to FLAG, and mitochondrial morphology of transfected cells was analysed by fluorescence microscopy. (D) Immunoblot analysis of cells described in (C). Whole-cell lysates were analysed by SDS–PAGE using antibodies against OPA1 and SLP-2. HSP70 served as a loading control (B, D). Data (A, C) represent the mean±s.d. of three independent experiments, each with >300 cells counted per condition. The blot (B, D) is representative of three independent experiments.
Figure 4
Figure 4
SLP-2 prevents OPA1 proteolysis during SIMH. (A) Immunoblot analysis of Opa1−/− MEFs stably expressing shSLP-2 or shLuc after transient transfection of FLAG-tagged rat OPA1 variant 1 (rOPA1-V1) and its non-cleavable deletion mutant (rOPA1-V1-ΔS1) at the indicated time points after CHX treatment. Whole-cell lysates were analysed by SDS–PAGE using antibodies against OPA1. (B) Quantification of mitochondrial morphology in Opa1−/− MEFs stably expressing shLuc or shSLP-2 after transient transfection of mitochondrial targeted YFP alone (not shown) or together with rOPA1-V1 and rOPA1-V1-ΔS1. Cells were exposed to 10 μM CHX for 8 h and YFP-positive cells were analysed by fluorescence microscopy (C). (D) Wild-type MEFs stably expressing shRNA against Luciferase (shLuc) or SLP-2 (shSLP-2) were treated with indicated concentrations of (1,10)-o-phenanthroline or 1 mM PMSF alone or in combination with 10 μM CHX. After 3 h, cells were harvested and whole-cell lysates were used for SDS–PAGE. (E) Mitochondrial morphology in Pbh2fl/fl MEFs (WT), Prohibitin 2 knockout (Phb2−/−) and Phb2−/− re-expressing prohibitin 2 (PHB2∷Phb2−/−) 6 h after exposure to 10 μM CHX. (F) Quantification of cells shown in (E). HSP70 served as a loading control (A, D). Data (B) represent the mean±s.d. of three independent experiments, each with >300 cells counted per condition.
Figure 5
Figure 5
SIMH promotes mitochondrial ATP production. (A, B) Measurement of total ATP levels in WT MEFs treated with Act D (3 μM), CHX (10 μM), UV (60 mJ/cm2), and STS (0.25 μM) (A), in SIMH competent (WT, Mfn2−/−, shLuc) and incompetent (Mfn1−/−, OPA1−/−, shSLP-2) cells (B) exposed to 10 μM CHX treatment for 6 h. (C–E) Measurement of mitochondrial ATP production. Cells were pretreated with 10 μM CHX for 6 h, followed by 10 mM methylpyruvate (MeP). Cells were harvested after 5, 10 or 15 min and total ATP levels assayed. In (C) ATP levels were also measured 15 min after co-addition of methylpyruvate+oligomycin (10 μM). Data represent the mean±s.e.m of at least three independent experiments. *P<0.05; **P<0.01; ***P<0.005; students t-test, unpaired, two-tailed.
Figure 6
Figure 6
SIMH is associated with ultrastructural changes. Ultrastructural analysis of WT, Mfn1−/−, hMFN1-HA:Mfn1−/−, OPA1−/−, mOPA1-V1:OPA1−/−, shLuc, and shSLP-2 MEFs. Cells were untreated (left part) or exposed to 10 μM CHX (right part) and fixed after 6 h for electron microscopy analysis. Scale bar, 1 μm.
Figure 7
Figure 7
Stress-induced mitochondrial hyperfusion protects against apoptosis. (A) Death of Mfn1−/− and hMFN1-HA:Mfn1−/− cells exposed to 3 μg/ml Act D or 60 mJ/cm2 UV-C. (B) Death of HeLa and MEF cells transiently transfected with shSLP-2 or shLuc after exposure to 60 mJ/cm2 UV-C. (C) Death of Mfn1−/− and hMFN1-HA:Mfn1−/− MEF cells exposed to 1 μM staurosporine. In all cases cells were harvested at the indicated time points and stained with annexin V FITC for flow-cytometric analysis. Data represent the mean±s.e.m of at least three independent experiments (*P<0.05; **P<0.01).
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References

    1. Alexander C, Votruba M, Pesch UE, Thiselton DL, Mayer S, Moore A, Rodriguez M, Kellner U, Leo-Kottler B, Auburger G, Bhattacharya SS, Wissinger B (2000) OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28. Nat Genet 26: 211–215 - PubMed
    1. Arnould T, Mercy L, Houbion A, Vankoningsloo S, Renard P, Pascal T, Ninane N, Demazy C, Raes M (2003) mtCLIC is up-regulated and maintains a mitochondrial membrane potential in mtDNA-depleted L929 cells. Faseb J 17: 2145–2147 - PubMed
    1. Baricault L, Segui B, Guegand L, Olichon A, Valette A, Larminat F, Lenaers G (2007) OPA1 cleavage depends on decreased mitochondrial ATP level and bivalent metals. Exp Cell Res 313: 3800–3808 - PubMed
    1. Bowes T, Gupta RS (2008) Novel mitochondrial extensions provide evidence for a link between microtubule-directed movement and mitochondrial fission. Biochem Biophys Res Commun 376: 40–45 - PubMed
    1. Breckenridge DG, Kang BH, Kokel D, Mitani S, Staehelin LA, Xue D (2008) Caenorhabditis elegans drp-1 and fis-2 regulate distinct cell-death execution pathways downstream of ced-3 and independent of ced-9. Mol Cell 31: 586–597 - PMC - PubMed

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