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. 2009 Mar;3(1):19-23.
doi: 10.1007/s12079-009-0044-8. Epub 2009 Mar 25.

Expression of CCN family of genes in human skin in vivo and alterations by solar-simulated ultraviolet irradiation

Taihao Quan  1 , Sharon Shin, Zhaoping Qin, Gary J Fisher
Affiliations

Expression of CCN family of genes in human skin in vivo and alterations by solar-simulated ultraviolet irradiation

Taihao Quan et al. J Cell Commun Signal. 2009 Mar.

Abstract

The CCN family of proteins is involved in diverse biological functions such as cell growth, adhesion, migration, angiogenesis, and regulation of extracellular matrix. We have investigated expression of CCN family genes and alternations induced by solar-simulated ultraviolet irradiation in human skin in vivo. Transcripts of all six CCN genes were expressed in human skin in vivo. CCN5 was most abundantly expressed followed by CCN2>CCN3>CCN1>CCN4>CCN6. Solar-simulated ultraviolet irradiation increased mRNA expression of CCN1 and CCN2. In contrast, mRNA levels of CCN3, CCN4, CCN5, and CCN6, were reduced. Knowledge gained from this study provides the foundation to explore the functional roles of CCN gene products in cutaneous biology and responses to solar ultraviolet irradiation.

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Figures

Fig. 1
Fig. 1
Expression of CCN genes in normal human skin in vivo. Full-thickness adult human skin samples from buttock were obtained, and total RNA was extracted as described in Methods. CCN members and 36B4 (internal control) mRNA levels were quantified using real-time RT-PCR. mRNA levels of CCN genes were normalized to mRNA levels of house keeping gene, 36B4. Data are expressed as mean±SEM, N = 6
Fig. 2
Fig. 2
UV irradiation alters CCN gene expression in human skin in vivo. Adult human buttock skin was exposed to 2 MED solar-simulated UV irradiation. Skin samples were obtained at indicated time points and total RNA was extracted. mRNA levels of CCN family members and 36B4 were quantified using real-time RT-PCR. mRNA levels of CCN genes were normalized to mRNA levels of house keeping gene, 36B4 (internal control). a CCN1/CYR61 b CCN2/CTGF c CCN3/NOV d CCN4/WISP1 e CCN5/WISP5 f CCN6/WISP3. Results are expressed as mean ± SEM, N = 6 for each time point, *p < 0.05
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