Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Dec;54(12):2655-62.
doi: 10.1007/s10620-008-0665-4.

Ultra-high performance liquid chromatography-mass spectrometry for the metabolomic analysis of urine in colorectal cancer

Yan-Lei Ma  1 , Huan-Long Qin, Wei-Jie Liu, Jia-Yuan Peng, Long Huang, Xiao-Ping Zhao, Yi-Yu Cheng
Affiliations

Ultra-high performance liquid chromatography-mass spectrometry for the metabolomic analysis of urine in colorectal cancer

Yan-Lei Ma et al. Dig Dis Sci. 2009 Dec.

Abstract

We report here the results of a pilot study in which ultra-high performance liquid chromatography/time-of- flight-mass spectrometry (UPLC/TOF-MS) and multivariate statistical analysis (supervised partial least squares discriminant analysis, PLS-DA) were applied for urinary metabolite profiling and data interpretation. The results of the PLS-DA indicated that the metabolic pattern as a whole was significantly different between the groups of preoperative colorectal cancer (CRC) patients, postoperative CRC patients, and healthy volunteers, respectively. The preoperative group of patients showed significantly increased levels of low-molecular weight compounds (LMC) MW 283 and MW 234 in comparison to the group of healthy volunteers group. After the operation, the levels of these two LMC significantly decreased. These preliminary results suggest that the UPLC-MS-based method coupled with pattern recognition will likely lead to procedures with the potential to be clinically applicable for the diagnosis of CRC and, consequently, to an improvement in patient prognosis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bioessays. 2006 May;28(5):534-45 - PubMed
    1. Electrophoresis. 2002 Dec;23(24):4104-9 - PubMed
    1. J Pharm Biomed Anal. 2005 Jun 15;38(2):337-43 - PubMed
    1. J Proteome Res. 2007 Feb;6(2):498-505 - PubMed
    1. J Proteome Res. 2005 Mar-Apr;4(2):591-8 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources