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. 1991 Sep;11(9):4398-404.
doi: 10.1128/mcb.11.9.4398-4404.1991.

High expression of a 3'----5' exonuclease activity is specific to B lymphocytes

A L Kenter  1 , J Tredup
Affiliations

High expression of a 3'----5' exonuclease activity is specific to B lymphocytes

A L Kenter et al. Mol Cell Biol. 1991 Sep.

Abstract

V(D)J joining, the immunoglobulin heavy-chain (IgH) class switch, and somatic hypermutation directed at variable regions are unique genetic recombination or mutation events which occur during B-cell differentiation. The enzymatic process directing and controlling these events remains obscure. An assay for exonucleolytic activity has been devised, and an exonuclease activity expressed at high levels in normal B lymphocytes has been detected. The high expression of this enzyme is specific to B lymphocytes and may be developmentally regulated. We have partially purified a B-cell-associated nuclease by column chromatography. Using this preparation, we have begun a rigorous analysis of its activity. This activity is a nonprocessive, 3'----5' exonuclease with a requirement for divalent cations. Our studies demonstrate that EDTA, poly(dI-dC), and glycerol are all inhibitory to B-cell-associated exonucleolytic activity. The exonuclease displays sequence preference but no sequence specificity when tested on a variety of native DNA substrates. This nuclease is distinct from other exonuclease activities previously described.

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References

    1. Eur J Immunol. 1986 Nov;16(11):1439-44 - PubMed
    1. Biochemistry. 1976 Jul 13;15(14):3114-21 - PubMed
    1. J Immunol Methods. 1987 Feb 26;97(1):111-7 - PubMed
    1. Exp Cell Res. 1986 Nov;167(1):241-51 - PubMed
    1. Nature. 1985 Aug 1-7;316(6027):412-8 - PubMed

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