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. 2008 Oct 10;375(1):16-21.
doi: 10.1016/j.bbrc.2008.07.067. Epub 2008 Jul 24.

Identification of novel retromer complexes in the mouse testis

Ekyune Kim  1 , Jae-Woong Lee, Dong-Chul Baek, Sang-Rae Lee, Myeong-Su Kim, Sang-Hyun Kim, Kazuhiko Imakawa, Kyu-Tae Chang
Affiliations

Identification of novel retromer complexes in the mouse testis

Ekyune Kim et al. Biochem Biophys Res Commun. .

Abstract

A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay using antibodies raised against each Vps component. Our results revealed that the retromer complex could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues.

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