Reactions of nitric oxide and oxygen with the regulator of fumarate and nitrate reduction, a global transcriptional regulator, during anaerobic growth of Escherichia coli
- PMID: 18433630
- DOI: 10.1016/S0076-6879(07)37011-0
Reactions of nitric oxide and oxygen with the regulator of fumarate and nitrate reduction, a global transcriptional regulator, during anaerobic growth of Escherichia coli
Abstract
The Escherichia coli fumarate and nitrate reductase (FNR) regulator protein is an important transcriptional regulator that controls the expression of a large regulon of more than 100 genes in response to changes in oxygen availability. FNR is active when it acquires a [4Fe-4S](2+) cluster under anaerobic conditions. The presence of the [4Fe-4S](2+) cluster promotes protein dimerization and site-specific DNA binding, facilitating activation or repression of target promoters. Oxygen is sensed by the controlled disassembly of the [4Fe-4S](2+) cluster, ultimately resulting in inactive, monomeric, apo-FNR. The FNR [4Fe-4S](2+) cluster is also sensitive to nitric oxide, such that under anaerobic conditions the protein is inactivated by nitrosylation of the iron-sulfur cluster, yielding a mixture of monomeric and dimeric dinitrosyl-iron cysteine species. This chapter describes some of the methods used to produce active [4Fe-4S] FNR protein and investigates the reaction of the [4Fe-4S](2+) cluster with nitric oxide and oxygen in vitro.
Similar articles
-
ClpXP-dependent proteolysis of FNR upon loss of its O2-sensing [4Fe-4S] cluster.J Mol Biol. 2005 Nov 25;354(2):220-32. doi: 10.1016/j.jmb.2005.09.066. Epub 2005 Oct 7. J Mol Biol. 2005. PMID: 16243354
-
Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S].FNR of Escherichia coli.FEBS J. 2005 Aug;272(16):4260-9. doi: 10.1111/j.1742-4658.2005.04840.x. FEBS J. 2005. PMID: 16098206
-
Superoxide destroys the [2Fe-2S]2+ cluster of FNR from Escherichia coli.Biochemistry. 2004 Jan 27;43(3):791-8. doi: 10.1021/bi0357053. Biochemistry. 2004. PMID: 14730984
-
Control of FNR function of Escherichia coli by O2 and reducing conditions.J Mol Microbiol Biotechnol. 2002 May;4(3):263-8. J Mol Microbiol Biotechnol. 2002. PMID: 11931557 Review.
-
Oxygen sensing by the global regulator, FNR: the role of the iron-sulfur cluster.FEMS Microbiol Rev. 1998 Dec;22(5):341-52. doi: 10.1111/j.1574-6976.1998.tb00375.x. FEMS Microbiol Rev. 1998. PMID: 9990723 Review.
Cited by
-
Fnr Negatively Regulates Prodigiosin Synthesis in Serratia sp. ATCC 39006 During Aerobic Fermentation.Front Microbiol. 2021 Sep 17;12:734854. doi: 10.3389/fmicb.2021.734854. eCollection 2021. Front Microbiol. 2021. PMID: 34603264 Free PMC article.
-
Three Pseudomonas putida FNR Family Proteins with Different Sensitivities to O2.J Biol Chem. 2015 Jul 3;290(27):16812-23. doi: 10.1074/jbc.M115.654079. Epub 2015 May 13. J Biol Chem. 2015. PMID: 25971977 Free PMC article.
-
Characterization of [4Fe-4S]-containing and cluster-free forms of Streptomyces WhiD.Biochemistry. 2009 Dec 29;48(51):12252-64. doi: 10.1021/bi901498v. Biochemistry. 2009. PMID: 19954209 Free PMC article.
-
The dpsA gene of Streptomyces coelicolor: induction of expression from a single promoter in response to environmental stress or during development.PLoS One. 2011;6(9):e25593. doi: 10.1371/journal.pone.0025593. Epub 2011 Sep 30. PLoS One. 2011. PMID: 21984935 Free PMC article.
-
Transcriptional regulation of bacterial virulence gene expression by molecular oxygen and nitric oxide.Virulence. 2014;5(8):794-809. doi: 10.4161/viru.27794. Epub 2014 Oct 31. Virulence. 2014. PMID: 25603427 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases