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Review
. 2002 Feb:Chapter 6:Unit 6.7.
doi: 10.1002/0471142700.nc0607s07.

Probing DNA structure with hydroxyl radicals

Thomas D Tullius  1
Affiliations
Review

Probing DNA structure with hydroxyl radicals

Thomas D Tullius. Curr Protoc Nucleic Acid Chem. 2002 Feb.

Abstract

The hydroxyl radical is a useful probe for studying the shape of the surface of a DNA molecule. Using this technique, fine details of DNA structure can potentially be revealed. This unit describes how to use the hydroxyl radical to generate a random cleavage pattern at the surface of the molecule, separate the broken DNA strands by polyacrylamide gel electrophoresis, and analyze the cleavage pattern to give an image of the surface of the molecule.

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