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. 2008 Jun 20;371(1):110-3.
doi: 10.1016/j.bbrc.2008.04.006. Epub 2008 Apr 10.

Interferon alfacon 1 inhibits SARS-CoV infection in human bronchial epithelial Calu-3 cells

Yohichi Kumaki  1 , Craig W Day, Miles K Wandersee, Bradley P Schow, Justin S Madsen, Dixon Grant, Jason P Roth, Donald F Smee, Lawrence M Blatt, Dale L Barnard
Affiliations

Interferon alfacon 1 inhibits SARS-CoV infection in human bronchial epithelial Calu-3 cells

Yohichi Kumaki et al. Biochem Biophys Res Commun. .

Abstract

The primary targets for SARS-CoV infection are the epithelial cells in the respiratory and intestinal tract. The angiotensin-converting enzyme 2 (ACE-2) has been identified as a functional receptor for SARS-CoV. ACE-2 has been shown to be expressed at the apical domain of polarized Calu-3 cells. In this report, interferon alfacon 1 was examined for inhibitory activities against SARS-CoV on human lung carcinoma epithelial Calu-3 cell line and the other three African green monkey kidney epithelial cell lines. Interferon alfacon 1 demonstrated significant antiviral activity in neutral red uptake assay and virus yield reduction assay. The data might provide an important insight into the mechanism of pathogenesis of SARS-CoV allowing further development of antiviral therapies for treating SARS infections.

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