CpG methylation inhibits binding of several sequence-specific DNA-binding proteins from pea, wheat, soybean and cauliflower
- PMID: 1831056
- DOI: 10.1007/BF00036811
CpG methylation inhibits binding of several sequence-specific DNA-binding proteins from pea, wheat, soybean and cauliflower
Abstract
To elucidate how methylation of specific sites in plant DNA might control transcription, we examined the effect of DNA methylation at CpG sequences on the binding of plant nuclear factors to an oligonucleotide duplex containing the consensus sequence for mammalian CREB (cAMP response element binding protein). CREB is part of the ATF (activating transcription factor) family of mammalian proteins specifically binding to 5'-TGACGTCA-3' and related sequences. Proteins recognizing the CREB-specific ligand were identified in nuclear extracts of pea seeds, wheat germ, cauliflower, and soybean leaves using electrophoretic mobility shift assays. Cytosine methylation inhibited binding of this protein in all these extracts, and so this sequence-specific DNA-binding activity is referred to as methylation-inhibited binding protein 1 (MIB-1). Sites somewhat similar to that of the CREB ligand are found in the upstream regions of a wheat histone H3 gene and tomato and pea ribulose 1,5-bisphosphate carboxylase genes. These sites were bound preferentially by distinct proteins that may be related to the previously described plant proteins HBP-1, HSBF, ASF-1, or GBF. Methylation of cytosine residues at these sites and at a site for MIB-1 located upstream of a soybean proline-rich protein gene also reduced specific binding with all the nuclear extracts tested. Similarly, substitution of the central CpG dinucleotide with TpG decreased binding.
Similar articles
-
A broad bean cDNA clone encoding a DNA-binding protein resembling mammalian CREB in its sequence specificity and DNA methylation sensitivity.Gene. 1992 Aug 15;117(2):169-78. doi: 10.1016/0378-1119(92)90726-6. Gene. 1992. PMID: 1386331
-
Characterization of DBPm, a plant protein that binds to DNA containing 5-methylcytosine.Biochim Biophys Acta. 1993 Feb 20;1172(1-2):108-16. doi: 10.1016/0167-4781(93)90276-j. Biochim Biophys Acta. 1993. PMID: 8439549
-
Wheat nuclear protein HBP-1 binds to the hexameric sequence in the promoter of various plant genes.Nucleic Acids Res. 1989 Dec 11;17(23):9707-17. doi: 10.1093/nar/17.23.9707. Nucleic Acids Res. 1989. PMID: 2602142 Free PMC article.
-
Effect of DNA methylation on dynamic properties of the helix and nuclear protein binding in the H-ras promoter.EXS. 1993;64:330-42. doi: 10.1007/978-3-0348-9118-9_14. EXS. 1993. PMID: 8418953 Review. No abstract available.
-
Effect of DNA methylation on the binding of vertebrate and plant proteins to DNA.EXS. 1993;64:145-68. doi: 10.1007/978-3-0348-9118-9_7. EXS. 1993. PMID: 8418948 Review. No abstract available.
Cited by
-
Hordein promoter methylation and transcriptional activity in wild-type and mutant barley endosperm.Mol Gen Genet. 1996 Apr 10;250(6):750-60. doi: 10.1007/BF02172987. Mol Gen Genet. 1996. PMID: 8628236
-
Genetic and Epigenetic Alterations in Barrett's Esophagus and Esophageal Adenocarcinoma.Gastroenterol Clin North Am. 2015 Jun;44(2):473-89. doi: 10.1016/j.gtc.2015.02.015. Epub 2015 Apr 1. Gastroenterol Clin North Am. 2015. PMID: 26021206 Free PMC article. Review.
-
The Key Role of DNA Methylation and Histone Acetylation in Epigenetics of Atherosclerosis.J Lipid Atheroscler. 2020 Sep;9(3):419-434. doi: 10.12997/jla.2020.9.3.419. Epub 2020 Sep 21. J Lipid Atheroscler. 2020. PMID: 33024734 Free PMC article. Review.
-
Childhood adversity and DNA methylation of genes involved in the hypothalamus-pituitary-adrenal axis and immune system: whole-genome and candidate-gene associations.Dev Psychopathol. 2012 Nov;24(4):1417-25. doi: 10.1017/S0954579412000806. Dev Psychopathol. 2012. PMID: 23062307 Free PMC article. Review.
-
Methylation of the Opaque2 box in zein genes is parent-dependent and affects O2 DNA binding activity in vitro.Plant Mol Biol. 2001 Jul;46(5):549-60. doi: 10.1023/a:1010686721797. Plant Mol Biol. 2001. PMID: 11516148
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials
Miscellaneous