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. 2008 Apr;82(8):4180-3.
doi: 10.1128/JVI.02596-07. Epub 2008 Feb 6.

Epstein-Barr virus nuclear antigen 1 does not cause lymphoma in C57BL/6J mice

Myung-Soo Kang  1 , Vishal Soni, Roderick Bronson, Elliott Kieff
Affiliations

Epstein-Barr virus nuclear antigen 1 does not cause lymphoma in C57BL/6J mice

Myung-Soo Kang et al. J Virol. 2008 Apr.

Abstract

To test whether transgenic Epstein-Barr virus nuclear antigen 1 (EBNA1) expression in C57BL/6 mouse lymphocytes causes lymphoma, EBNA1 expressed in three FVB lineages at two or three times the level of latent infection was crossed up to six successive times into C57BL/6J mice. After five or six crosses, 14/36, (38%) EBNA1 transgenic mice, 11/31 (36%) littermate EBNA1-negative controls, and 9/25 (36%) inbred C57BL/6J mice housed in the same facility had lymphoma. These data indicate that EBNA1 does not significantly increase lymphoma prevalence in C57BL/6J mice.

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Figures

FIG. 1.
FIG. 1.
The EBNA1 transgene in FVB cross C57BL/6J mouse spleen cells. (A and B) After the second cross of the EBNA1 transgene from FVB to C57BL/6J mice, spleen extracts from each of three Flag-tagged EBNA1 transgenic lineages (58, 68, and 73) or control C57BL/6J mice (C) were assayed for protein with Bradford reagent and dissolved in sodium dodecyl sulfate sample buffer at 95°C, and 8, 24, or 80 μg of protein was loaded onto a denaturing polyacrylamide gel, along with similar amounts of protein from IB4 LCLs (LCL), from Flag-tagged EBNA1-expressing BJAB cells (E1), or from BJAB cells (B), and was blotted with anti-EBNA1 or anti-alpha tubulin antibody (23). (C) Spleen extracts were incubated with labeled family of repeats probe in the absence or presence of 500-fold excess unlabeled wild-type (WT) or mutant (MT) competitor or anti-EBNA1 (αE1), anti-Flag (αFL), or Ig isotype (Ig) control antibody (23). BSA, bovine serum albumin.
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