Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Mar;310(1-2):77-83.
doi: 10.1007/s11010-007-9667-3. Epub 2007 Dec 9.

Mono ADP-ribosylation inhibitors prevent inflammatory cytokine release in alveolar epithelial cells

Mariangela Del Vecchio  1 , Enrico Balducci
Affiliations

Mono ADP-ribosylation inhibitors prevent inflammatory cytokine release in alveolar epithelial cells

Mariangela Del Vecchio et al. Mol Cell Biochem. 2008 Mar.

Abstract

A549, a type II alveolar epithelial cell line stimulated with LPS (10 mug/ml), released high levels of the inflammatory cytokines IL-6 and IL-8. Here, we have investigated whether ADP-ribosylation inhibitors block the LPS-triggered cytokine release in epithelial cells. When coincubating A549 with LPS and meta-iodobenzylguanidine or novobiocin, selective arginine-dependent ART-inhibitors, the release of IL-6 and IL-8 was inhibited in a concentration-dependent manner. This effect has been linked with the presence of a functionally active arginine ADP-ribosylating enzyme on the cell surface. To this aim, we amplified by RT-PCR the ART1 transcript and identified four ADP-ribosylated proteins likely substrate for ART1. The mechanism behind the cytokine inhibition in epithelial cells seems to be correlated with the presence of ART1, which behaves as an essential positive regulator of inflammatory cytokines. This novel observation indicates this enzyme as well as other novobiocin/MIBG sensitive ARTs as potential targets for the development of new therapeutic strategies.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Eur J Biochem. 2003 Jan;270(1):101-10 - PubMed
    1. Mol Cell Biochem. 2002 Apr;233(1-2):127-32 - PubMed
    1. Int J Mol Med. 1998 Feb;1(2):367-78 - PubMed
    1. Annu Rev Biochem. 1985;54:73-100 - PubMed
    1. J Biol Chem. 1992 Jan 25;267(3):1569-75 - PubMed

Publication types

MeSH terms

LinkOut - more resources