Three-dimensional structure of the respiratory chain supercomplex I1III2IV1 from bovine heart mitochondria
- PMID: 17927210
- DOI: 10.1021/bi700983h
Three-dimensional structure of the respiratory chain supercomplex I1III2IV1 from bovine heart mitochondria
Abstract
The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV (I1III2IV1). Within this 3D map the positions and orientations of all the individual complexes in the supercomplex were determined unambiguously. Furthermore, the ubiquinone and cytochrome c binding sites of each complex in the supercomplex could be located. The mobile electron carrier binding site of each complex was found to be in proximity to the binding site of the succeeding complex in the respiratory chain. This provides structural evidence for direct substrate channeling in the supercomplex assembly with short diffusion distances for the mobile electron carriers.
Similar articles
-
Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1.EMBO J. 2011 Sep 9;30(22):4652-64. doi: 10.1038/emboj.2011.324. EMBO J. 2011. PMID: 21909073 Free PMC article.
-
Architecture of active mammalian respiratory chain supercomplexes.J Biol Chem. 2006 Jun 2;281(22):15370-5. doi: 10.1074/jbc.M513525200. Epub 2006 Mar 20. J Biol Chem. 2006. PMID: 16551638
-
Respiratory complexes III and IV are not essential for the assembly/stability of complex I in fungi.J Mol Biol. 2009 Mar 27;387(2):259-69. doi: 10.1016/j.jmb.2008.12.025. Epub 2008 Dec 24. J Mol Biol. 2009. PMID: 19111556
-
Structural organization of the mitochondrial respiratory chain.Ital J Biochem. 2003 Mar;52(1):58-61. Ital J Biochem. 2003. PMID: 12833641 Review.
-
Three-dimensional structure and functions of bovine heart mitochondrial cytochrome bc1 complex.Biofactors. 1998;8(3-4):187-9. Biofactors. 1998. PMID: 9914817 Review. No abstract available.
Cited by
-
Lipid functions in cytochrome bc complexes: an odd evolutionary transition in a membrane protein structure.Philos Trans R Soc Lond B Biol Sci. 2012 Dec 19;367(1608):3406-11. doi: 10.1098/rstb.2012.0058. Philos Trans R Soc Lond B Biol Sci. 2012. PMID: 23148267 Free PMC article.
-
Mutations in mitochondrial complex III uniquely affect complex I in Caenorhabditis elegans.J Biol Chem. 2010 Dec 24;285(52):40724-31. doi: 10.1074/jbc.M110.159608. Epub 2010 Oct 22. J Biol Chem. 2010. PMID: 20971856 Free PMC article.
-
Supramolecular organization of the respiratory chain in Neurospora crassa mitochondria.Eukaryot Cell. 2007 Dec;6(12):2391-405. doi: 10.1128/EC.00149-07. Epub 2007 Sep 14. Eukaryot Cell. 2007. PMID: 17873079 Free PMC article.
-
Emerging Roles in the Biogenesis of Cytochrome c Oxidase for Members of the Mitochondrial Carrier Family.Front Cell Dev Biol. 2019 Jan 31;7:3. doi: 10.3389/fcell.2019.00003. eCollection 2019. Front Cell Dev Biol. 2019. PMID: 30766870 Free PMC article. Review.
-
Respiratory chain supercomplexes: Structures, function and biogenesis.Semin Cell Dev Biol. 2018 Apr;76:179-190. doi: 10.1016/j.semcdb.2017.07.021. Epub 2017 Jul 23. Semin Cell Dev Biol. 2018. PMID: 28743641 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources