doi: 10.1038/nmeth1060.
Epub 2007 Aug 26.
Higher-energy C-trap dissociation for peptide modification analysis
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- PMID: 17721543
- DOI: 10.1038/nmeth1060
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Higher-energy C-trap dissociation for peptide modification analysis
Nat Methods.
2007 Sep.
Abstract
Peptide sequencing is the basis of mass spectrometry-driven proteomics. Here we show that in the linear ion trap-orbitrap mass spectrometer (LTQ Orbitrap) peptide ions can be efficiently fragmented by high-accuracy and full-mass-range tandem mass spectrometry (MS/MS) via higher-energy C-trap dissociation (HCD). Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. Additionally we show that an added octopole collision cell facilitates de novo sequencing.
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