The patch clamp technique: principles and technical considerations
- PMID: 17689466
- DOI: 10.1016/j.jvc.2007.02.001
The patch clamp technique: principles and technical considerations
Abstract
The development of techniques that allow the high fidelity measurement of small scale ionic currents ushered in a new era of investigation into the role of ion channels in the physiologic and pathophysiologic function of excitable tissue. Based upon the formation of a high resistance (gigaohm) seal with the membrane of the cell being studied, these "patch clamp" techniques have improved our understanding of a wide variety of cardiac disease states with respect to diagnosis, treatment and prognosis. This review outlines the basic principles underlying the patch clamp technique, including the properties of biological membranes and ion channels, and provides an elementary summary of its application to the recording of cardiac ionic currents, with a particular focus on issues related to myocyte isolation, electrode manufacturing and the voltage clamp configuration.
Similar articles
-
[The use of thin slices of myocardium for recording the currents across single ion channels].Fiziol Zh (1978). 1991 Mar-Apr;37(2):119-22. Fiziol Zh (1978). 1991. PMID: 1711482 Russian.
-
A new model system for investigation of ionic channels in filamentous fungi: evidence for existence of two K+-permeable ionic channels in Phycomyces blakesleeanus.Ann N Y Acad Sci. 2005 Jun;1048:491-5. doi: 10.1196/annals.1342.075. Ann N Y Acad Sci. 2005. PMID: 16154984
-
Advances in patch clamp technique: towards higher quality and quantity.Gen Physiol Biophys. 2012 Jun;31(2):131-40. doi: 10.4149/gpb_2012_016. Gen Physiol Biophys. 2012. PMID: 22781816 Review.
-
[Advancement of patch-clamp technique and its application in drug high-throughput screening].Sheng Li Ke Xue Jin Zhan. 2005 Apr;36(2):125-9. Sheng Li Ke Xue Jin Zhan. 2005. PMID: 16222971 Review. Chinese.
-
["Micro-lead" technic for recording ion currents through the membranes of individual myocardial cells].Biull Eksp Biol Med. 1981 Dec;92(12):759-61. Biull Eksp Biol Med. 1981. PMID: 6275930 Russian.
Cited by
-
Microfluidic impedance flow cytometry enabling high-throughput single-cell electrical property characterization.Int J Mol Sci. 2015 Apr 29;16(5):9804-30. doi: 10.3390/ijms16059804. Int J Mol Sci. 2015. PMID: 25938973 Free PMC article. Review.
-
Case Report: Functional Investigation of an Undescribed Missense Variant Affecting Splicing in a Patient With Dravet Syndrome.Front Neurol. 2021 Dec 6;12:761892. doi: 10.3389/fneur.2021.761892. eCollection 2021. Front Neurol. 2021. PMID: 34938262 Free PMC article.
-
Porous Graphene Oxide Decorated Ion Selective Electrode for Observing Across-Cytomembrane Ion Transport.Sensors (Basel). 2020 Jun 21;20(12):3500. doi: 10.3390/s20123500. Sensors (Basel). 2020. PMID: 32575810 Free PMC article.
-
Electrophysiological evaluation of the effect of peptide toxins on voltage-gated ion channels: a scoping review on theoretical and methodological aspects with focus on the Central and South American experience.J Venom Anim Toxins Incl Trop Dis. 2024 Sep 2;30:e20230048. doi: 10.1590/1678-9199-JVATITD-2023-0048. eCollection 2024. J Venom Anim Toxins Incl Trop Dis. 2024. PMID: 39263598 Free PMC article. Review.
-
A look into retinal organoids: methods, analytical techniques, and applications.Cell Mol Life Sci. 2021 Oct;78(19-20):6505-6532. doi: 10.1007/s00018-021-03917-4. Epub 2021 Aug 22. Cell Mol Life Sci. 2021. PMID: 34420069 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
