Quantitative morphological signatures define local signaling networks regulating cell morphology
- PMID: 17588932
- DOI: 10.1126/science.1140324
Quantitative morphological signatures define local signaling networks regulating cell morphology
Abstract
Although classical genetic and biochemical approaches have identified hundreds of proteins that function in the dynamic remodeling of cell shape in response to upstream signals, there is currently little systems-level understanding of the organization and composition of signaling networks that regulate cell morphology. We have developed quantitative morphological profiling methods to systematically investigate the role of individual genes in the regulation of cell morphology in a fast, robust, and cost-efficient manner. We analyzed a compendium of quantitative morphological signatures and described the existence of local signaling networks that act to regulate cell protrusion, adhesion, and tension.
Similar articles
-
Involvement of Rac and Rho signaling in cancer cell motility in 3D substrates.Oncogene. 2009 Apr 2;28(13):1570-83. doi: 10.1038/onc.2009.2. Epub 2009 Feb 23. Oncogene. 2009. PMID: 19234490
-
Regulatory network for cell shape changes during Drosophila ventral furrow formation.J Theor Biol. 2006 Mar 7;239(1):49-62. doi: 10.1016/j.jtbi.2005.07.011. Epub 2005 Sep 2. J Theor Biol. 2006. PMID: 16139845
-
Plasticity of epithelial cell shape in response to upstream signals: a whole-organism study using transgenic Hydra.Zoology (Jena). 2009;112(3):185-94. doi: 10.1016/j.zool.2008.09.002. Epub 2009 Feb 7. Zoology (Jena). 2009. PMID: 19201587
-
Robust signaling networks of the adipose secretome.Trends Endocrinol Metab. 2009 Jan;20(1):1-7. doi: 10.1016/j.tem.2008.08.006. Epub 2008 Oct 17. Trends Endocrinol Metab. 2009. PMID: 18930409 Review.
-
Quantitative in vivo microscopy: the return from the 'omics'.Curr Opin Biotechnol. 2006 Oct;17(5):501-10. doi: 10.1016/j.copbio.2006.07.005. Epub 2006 Aug 8. Curr Opin Biotechnol. 2006. PMID: 16899361 Review.
Cited by
-
Systematic data analysis pipeline for quantitative morphological cell phenotyping.Comput Struct Biotechnol J. 2024 Jul 14;23:2949-2962. doi: 10.1016/j.csbj.2024.07.012. eCollection 2024 Dec. Comput Struct Biotechnol J. 2024. PMID: 39104709 Free PMC article. Review.
-
A Novel Nanosafety Approach Using Cell Painting, Metabolomics, and Lipidomics Captures the Cellular and Molecular Phenotypes Induced by the Unintentionally Formed Metal-Based (Nano)Particles.Cells. 2023 Jan 11;12(2):281. doi: 10.3390/cells12020281. Cells. 2023. PMID: 36672217 Free PMC article.
-
A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.Cell. 2011 Apr 29;145(3):470-82. doi: 10.1016/j.cell.2011.03.037. Cell. 2011. PMID: 21529718 Free PMC article.
-
RNAi screening: new approaches, understandings, and organisms.Wiley Interdiscip Rev RNA. 2012 Mar-Apr;3(2):145-58. doi: 10.1002/wrna.110. Epub 2011 Sep 22. Wiley Interdiscip Rev RNA. 2012. PMID: 21953743 Free PMC article. Review.
-
Ultra high content image analysis and phenotype profiling of 3D cultured micro-tissues.PLoS One. 2014 Oct 7;9(10):e109688. doi: 10.1371/journal.pone.0109688. eCollection 2014. PLoS One. 2014. PMID: 25289886 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases