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. 2007 Jul 19;448(7151):318-24.
doi: 10.1038/nature05944. Epub 2007 Jun 6.

In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state

Marius Wernig  1 , Alexander Meissner, Ruth Foreman, Tobias Brambrink, Manching Ku, Konrad Hochedlinger, Bradley E Bernstein, Rudolf Jaenisch
Affiliations

In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state

Marius Wernig et al. Nature. .

Abstract

Nuclear transplantation can reprogramme a somatic genome back into an embryonic epigenetic state, and the reprogrammed nucleus can create a cloned animal or produce pluripotent embryonic stem cells. One potential use of the nuclear cloning approach is the derivation of 'customized' embryonic stem (ES) cells for patient-specific cell treatment, but technical and ethical considerations impede the therapeutic application of this technology. Reprogramming of fibroblasts to a pluripotent state can be induced in vitro through ectopic expression of the four transcription factors Oct4 (also called Oct3/4 or Pou5f1), Sox2, c-Myc and Klf4. Here we show that DNA methylation, gene expression and chromatin state of such induced reprogrammed stem cells are similar to those of ES cells. Notably, the cells-derived from mouse fibroblasts-can form viable chimaeras, can contribute to the germ line and can generate live late-term embryos when injected into tetraploid blastocysts. Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.

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Comment in

  • Stem cells: the magic brew.
    Rossant J. Rossant J. Nature. 2007 Jul 19;448(7151):260-2. doi: 10.1038/448260a. Nature. 2007. PMID: 17637646 No abstract available.

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