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. 2007 Apr;13(4):620-2.
doi: 10.3201/eid1304.061390.

Human sapovirus in clams, Japan

Affiliations

Human sapovirus in clams, Japan

Grant S Hansman et al. Emerg Infect Dis. 2007 Apr.

Abstract

Human sapovirus was detected in 4 of 57 clam packages by reverse transcription-PCR and sequence analysis. This represents the first finding of sapovirus contamination in food. Closely matching sequences have been detected in stool specimens from patients with gastroenteritis in Japan, which indicates a possible food-to-human transmission link.

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Figures

Figure
Figure
Phylogenetic analysis of sapovirus capsid sequences (≈300 nt) showing the different genogroups and clusters. Numbers on each branch indicate bootstrap values for the genotype. Bootstrap values of ≥950 were considered statistically significant for the grouping. The scale represents nucleotide substitutions per site. GenBank accession nos. for the reference strains are as follows: Arg39, AY289803; C12, AY603425; Chiba/010598F, AJ412825; Chiba000496F, AJ412800; Cruise ship, AY289804; Ehime643, DQ366345; Ehime1107, DQ058829; Houston27, U95644; Manchester, X86560; Mc2, AY237419; Mc10, AY237420; Mex340, AF435812; Parkville, U73124; Cowden, AF182760; Potsdam, AF294739; Sapporo, U65427; Stockholm, AF194182; SW278, DQ125333; water samples, DQ915088–DQ915094; and Yokote, AB253740. Boldface represents sequences detected in this study.

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