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. 2007 Aug;26(8):1283-8.
doi: 10.1007/s00299-007-0358-z. Epub 2007 Apr 24.

Transgenic Nicotiana benthamiana plants resistant to cucumber green mottle mosaic virus based on RNA silencing

Shinichiro Kamachi  1 , Atsuko Mochizuki, Masamichi Nishiguchi, Yutaka Tabei
Affiliations

Transgenic Nicotiana benthamiana plants resistant to cucumber green mottle mosaic virus based on RNA silencing

Shinichiro Kamachi et al. Plant Cell Rep. 2007 Aug.

Abstract

RNA silencing technology was used to confer resistance to cucumber green mottle mosaic virus (CGMMV). Nicotiana benthamiana was transformed with a transgene designed to produce an inverted repeat RNA containing CGMMV-coat protein gene (CP) sequences, which were separated by an intron sequence, under the control of the cauliflower mosaic virus 35S promoter. We attempted to confirm the resistance of seven independent transgenic lines; five lines showed resistance to CGMMV infection. The systemic spread of virus was prevented after the inoculation of CGMMV, and the CP-specific short interfering RNA (siRNA) was detected in resistant lines. Thus, the resistance against CGMMV through RNA silencing is strong and efficient.

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