Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006;26(6):709-12.
doi: 10.1007/s11596-006-0622-4.

Neurocyte apoptosis and expressions of caspase-3 and Fas after spinal cord injury and their implication in rats

Jinguang Wang  1 , Qixin Zheng, Ming Zhao, Xiaodong Guo
Affiliations

Neurocyte apoptosis and expressions of caspase-3 and Fas after spinal cord injury and their implication in rats

Jinguang Wang et al. J Huazhong Univ Sci Technolog Med Sci. 2006.

Abstract

To study the expression of neurocyte apoptosis and the changes of caspase-3 and Fas after spinal cord injury (SCI) in rats, improved Allen's method was used to make model of acute SCI at the level of T9 and T10. The animals were divided into six groups: a control group and 5 injury groups. The segments of injured spinal cords were taken 6, 24, 48 h and 7, 15 days after injury for morphological studies, including HE staining, Hoechst33258 staining and TUNEL labeling. The expression of caspase-3 was detected by immunohistochemical staining and RT-PCR. TUNEL-positive cells began to appear in the compression region 6 h after the injury, mostly located in the gray matter. TUNEL-positive cells were found in both gray and white matter, reaching a peak at the 3rd day. They began to decrease at the 7th day, distributed mostly in the white matter. Fas increased at the 6th h and peaked at the 3rd day. Caspase-3 mRNA increased at the 6th h, peaking 48 h after the trauma, and decreased after 7 days. The protein expression of caspase-3, as revealed by immunohistochemical staining, was similar to TUNEL in time. It is concluded that apoptosis takes place after spinal cord injury, and caspase-3 mRNA and protein expressions were enhanced in the apoptosis. The expression of caspase-3 has a positive correlation with Fas expression.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Spinal Cord. 2000 Aug;38(8):449-72 - PubMed
    1. J Neurotrauma. 1998 Jul;15(7):459-72 - PubMed
    1. Adv Physiol Educ. 2002 Dec;26(1-4):238-55 - PubMed
    1. Nat Med. 1997 Jan;3(1):73-6 - PubMed
    1. J Neurosurg. 2001 Apr;94(2 Suppl):257-64 - PubMed