Pre-B lymphocyte-specific transcriptional control of the mouse VpreB gene
- PMID: 1730257
- DOI: 10.1002/eji.1830220106
Pre-B lymphocyte-specific transcriptional control of the mouse VpreB gene
Abstract
The VpreB genes, which encode surrogate immunoglobulin light chain molecules, are expressed as RNA almost exclusively in pre-B cells. We have investigated the transcriptional control mechanisms which are responsible for the pre-B cell-specific RNA expression of the mouse VpreB1 and VpreB2 genes. Nuclear run-on analyses demonstrate that the pre-B cell-specific expression of both VpreB genes is controlled primarily at the level of initiation of transcription. S1 nuclease protection-mapping defined two or three major start sites of transcription for the VpreB genes. To find a promoter and other potential cis-acting regulatory elements, a 700-bp fragment 5' of the transcription start sites of the VpreB1 gene was used in gene transfer experiments and found to act as a promoter in pre-B lymphocytes. Deletion experiments showed that 191 bp upstream of the most 5' transcription start site is required for the pre-B cell promoter activity. DNA sequence analysis of the 5' region of the mouse VpreB1, VpreB2 and human VpreB genes reveal that this region of approximately 200 bp is strongly conserved. This 200-bp promoter region contains several conserved nucleotide sequence motifs which may act to mediate the pre-B cell-specific transcription of the VpreB genes.
Similar articles
-
The murine VpreB1 and VpreB2 genes both encode a protein of the surrogate light chain and are co-expressed during B cell development.Eur J Immunol. 1996 Apr;26(4):906-13. doi: 10.1002/eji.1830260428. Eur J Immunol. 1996. PMID: 8625987
-
The 14.1 surrogate light chain promoter has lineage- and stage-restricted activity.J Immunol. 1997 Feb 15;158(4):1681-91. J Immunol. 1997. PMID: 9029104
-
A pre-B- and B cell-specific DNA-binding protein, EBB-1, which binds to the promoter of the VpreB1 gene.Eur J Immunol. 1992 Jan;22(1):37-43. doi: 10.1002/eji.1830220107. Eur J Immunol. 1992. PMID: 1730258
-
The lambda5-VpreB1 locus--a model system for studying gene regulation during early B cell development.Semin Immunol. 2005 Apr;17(2):121-7. doi: 10.1016/j.smim.2005.01.004. Semin Immunol. 2005. PMID: 15737573 Review.
-
Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells.Immunol Rev. 2000 Jun;175:33-46. Immunol Rev. 2000. PMID: 10933589 Review.
Cited by
-
Silencer elements controlling the B29 (Igbeta) promoter are neither promoter- nor cell-type-specific.Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12314-9. doi: 10.1073/pnas.94.23.12314. Proc Natl Acad Sci U S A. 1997. PMID: 9356446 Free PMC article.
-
A novel gene product associated with mu chains in immature B cells.EMBO J. 1993 May;12(5):1827-34. doi: 10.1002/j.1460-2075.1993.tb05831.x. EMBO J. 1993. PMID: 8491176 Free PMC article.
-
Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells.Mol Cell Biol. 2002 Jul;22(13):4771-80. doi: 10.1128/MCB.22.13.4771-4780.2002. Mol Cell Biol. 2002. PMID: 12052884 Free PMC article.
-
Mutations in the human lambda5/14.1 gene result in B cell deficiency and agammaglobulinemia.J Exp Med. 1998 Jan 5;187(1):71-7. doi: 10.1084/jem.187.1.71. J Exp Med. 1998. PMID: 9419212 Free PMC article.
-
New nucleotide sequence data on the EMBL File Server.Nucleic Acids Res. 1992 Oct 25;20(20):5495-515. doi: 10.1093/nar/20.20.5495. Nucleic Acids Res. 1992. PMID: 1437578 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases
