Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex
- PMID: 17202194
- DOI: 10.1093/jb/mvm034
Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex
Abstract
Members of the family of WASP-family Verprolin homologous proteins (WAVEs) activate the Arp2/3 complex to induce actin polymerization. The WAVE family comprises three proteins, namely, WAVE1, WAVE2 and WAVE3. Among them, WAVE2 is crucial for activation of the Arp2/3 complex for the formation of branched actin filaments in lamellipodia. Activation of mitogen-activated protein (MAP) kinase signalling results in the phosphorylation of the WAVE family proteins; however, which of the three WAVE proteins is phosphorylated is unclear. We found that in vitro WAVE2 is directly phosphorylated by a MAP kinase, i.e. extracellular signal-regulated kinase (ERK) 2. The proline-rich region and the verprolin, cofilin and acidic (VCA) region of WAVE2 were phosphorylated. Interestingly, the phosphorylated VCA region had a higher affinity for the Arp2/3 complex. However, the phosphorylation of the VCA region resulted in reduced induction of Arp2/3-mediated actin polymerization in vitro. The role of the phosphorylation of the proline-rich region was not determined.
Similar articles
-
From N-WASP to WAVE: key molecules for regulation of cortical actin organization.Novartis Found Symp. 2005;269:3-10; discussion 10-5, 30-4 passim. Novartis Found Symp. 2005. PMID: 16355531
-
Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex.Biochem Biophys Res Commun. 1999 Jun 24;260(1):296-302. doi: 10.1006/bbrc.1999.0894. Biochem Biophys Res Commun. 1999. PMID: 10381382
-
The WASP-WAVE protein network: connecting the membrane to the cytoskeleton.Nat Rev Mol Cell Biol. 2007 Jan;8(1):37-48. doi: 10.1038/nrm2069. Nat Rev Mol Cell Biol. 2007. PMID: 17183359 Review.
-
Activation of Arp2/3 complex-dependent actin polymerization by plant proteins distantly related to Scar/WAVE.Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16379-84. doi: 10.1073/pnas.0407392101. Epub 2004 Nov 8. Proc Natl Acad Sci U S A. 2004. PMID: 15534215 Free PMC article.
-
Protein complexes regulating Arp2/3-mediated actin assembly.Curr Opin Cell Biol. 2006 Feb;18(1):4-10. doi: 10.1016/j.ceb.2005.12.003. Epub 2005 Dec 15. Curr Opin Cell Biol. 2006. PMID: 16343889 Review.
Cited by
-
Phosphoregulation of the WAVE regulatory complex and signal integration.Semin Cell Dev Biol. 2013 Apr;24(4):272-9. doi: 10.1016/j.semcdb.2013.01.007. Epub 2013 Jan 24. Semin Cell Dev Biol. 2013. PMID: 23354023 Free PMC article. Review.
-
Extracellular Signalling Modulates Scar/WAVE Complex Activity through Abi Phosphorylation.Cells. 2021 Dec 10;10(12):3485. doi: 10.3390/cells10123485. Cells. 2021. PMID: 34943993 Free PMC article.
-
Dynamics of the Rho-family small GTPases in actin regulation and motility.Cell Adh Migr. 2011 Mar-Apr;5(2):170-80. doi: 10.4161/cam.5.2.14403. Epub 2011 Mar 1. Cell Adh Migr. 2011. PMID: 21178402 Free PMC article. Review.
-
WAVE2 is regulated by multiple phosphorylation events within its VCA domain.Cell Motil Cytoskeleton. 2009 Jan;66(1):36-47. doi: 10.1002/cm.20323. Cell Motil Cytoskeleton. 2009. PMID: 19012317 Free PMC article.
-
WASp family verprolin-homologous protein-2 (WAVE2) and Wiskott-Aldrich syndrome protein (WASp) engage in distinct downstream signaling interactions at the T cell antigen receptor site.J Biol Chem. 2014 Dec 12;289(50):34503-19. doi: 10.1074/jbc.M114.591685. Epub 2014 Oct 23. J Biol Chem. 2014. PMID: 25342748 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
