Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Nov;115(3):587-96.
doi: 10.1083/jcb.115.3.587.

A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription

M B Roth  1 , A M Zahler, J A Stolk
Affiliations

A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription

M B Roth et al. J Cell Biol. 1991 Nov.

Abstract

An antibody was identified previously that recognizes sites of polymerase II transcription on lampbrush chromosomes, puffs on polytene chromosomes, and many small granules in the nucleoplasm of all cells tested. This antibody binds a conserved family of phosphorylated polypeptides in vertebrate and invertebrate cells. We developed a method for purifying these proteins that involves differential solubility in MgCl2. We isolated a Drosophila cDNA encoding one of the proteins using information obtained from microsequencing. In vivo expression studies show that this protein is concentrated on sites of polymerase II transcription and that it is highly phosphorylated. The protein shares a high degree of homology with proteins involved in alternative splicing of pre-mRNA suggesting the possibility that this protein plays a role in pre-mRNA splicing.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8 - PubMed
    1. Mol Cell Biol. 1985 Dec;5(12):3610-6 - PubMed
    1. Cell. 1990 Jul 13;62(1):35-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 Jan;87(2):826-30 - PubMed
    1. Methods Enzymol. 1990;183:111-32 - PubMed

Publication types

MeSH terms