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. 1991 Aug 30;179(1):707-12.
doi: 10.1016/0006-291x(91)91430-k.

Phosphorylase kinase activity in I/strain neonatal skeletal muscle with a deficiency in alpha/alpha' subunit mRNAs

P K Bender  1
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Phosphorylase kinase activity in I/strain neonatal skeletal muscle with a deficiency in alpha/alpha' subunit mRNAs

P K Bender. Biochem Biophys Res Commun. .

Abstract

In the adult I/LnJ mouse skeletal muscle, phosphorylase kinase activity is 0.2% of that in normal. This deficiency results from a paucity of mRNA's for the phosphorylase kinase regulatory subunit- alpha and its isoform alpha'. However, in the I/LnJ neonatal skeletal muscle phosphorylase kinase activity is 20-25% of that in normal. During the first two months of development this activity decreases while in normal tissue it increases. The developmental differences in the magnitude of the I/LnJ deficiency indicate the possibility of stage specific mechanisms regulating the accumulation of alpha/alpha' mRNAs. To investigate this possibility, the abundance of alpha/alpha' mRNAs and of the catalytic subunit, gamma, mRNAs were compared by Northern Blot analysis. The results demonstrate that neonatal and adult I/LnJ skeletal muscle have a similar paucity of alpha/alpha' mRNAs whereas accumulation of gamma mRNAs is not significantly different from normal.

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