doi: 10.1016/j.cell.2006.09.037.
The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome
Affiliations
- PMID: 17110332
- DOI: 10.1016/j.cell.2006.09.037
Item in Clipboard
The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome
Cell.
.
Free article
Abstract
The ribosomal elongation cycle describes a series of reactions prolonging the nascent polypeptide chain by one amino acid and driven by two universal elongation factors termed EF-Tu and EF-G in bacteria. Here we demonstrate that the extremely conserved LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis. LepA has the unique function of back-translocating posttranslocational ribosomes, and the results suggest that it recognizes ribosomes after a defective translocation reaction and induces a back-translocation, thus giving EF-G a second chance to translocate the tRNAs correctly. We suggest renaming LepA as elongation factor 4 (EF4).
Similar articles
-
The ribosomal stalk binds to translation factors IF2, EF-Tu, EF-G and RF3 via a conserved region of the L12 C-terminal domain.J Mol Biol. 2007 Jan 12;365(2):468-79. doi: 10.1016/j.jmb.2006.10.025. Epub 2006 Oct 27. J Mol Biol. 2007. PMID: 17070545
-
Single-molecule structural dynamics of EF-G--ribosome interaction during translocation.Biochemistry. 2007 Sep 25;46(38):10767-75. doi: 10.1021/bi700657d. Epub 2007 Aug 30. Biochemistry. 2007. PMID: 17727272
-
Mutagenesis of glutamine 290 in Escherichia coli and mitochondrial elongation factor Tu affects interactions with mitochondrial aminoacyl-tRNAs and GTPase activity.Biochemistry. 2004 Jun 8;43(22):6917-27. doi: 10.1021/bi036068j. Biochemistry. 2004. PMID: 15170329
-
Ribosomal translocation: LepA does it backwards.Curr Biol. 2007 Feb 20;17(4):R136-9. doi: 10.1016/j.cub.2006.12.029. Curr Biol. 2007. PMID: 17307049 Review.
-
Possible evolution of factors involved in protein biosynthesis.Acta Biochim Pol. 1998;45(4):883-94. Acta Biochim Pol. 1998. PMID: 10397336 Review.
Cited by
-
Ribosome profiling reveals downregulation of UMP biosynthesis as the major early response to phage infection.Microbiol Spectr. 2024 Apr 2;12(4):e0398923. doi: 10.1128/spectrum.03989-23. Epub 2024 Mar 7. Microbiol Spectr. 2024. PMID: 38451091 Free PMC article.
-
Lacticaseibacillus paracasei AD22 Stress Response in Brined White Cheese Matrix: In Vitro Probiotic Profiles and Molecular Characterization.Probiotics Antimicrob Proteins. 2024 Feb 29. doi: 10.1007/s12602-024-10216-4. Online ahead of print. Probiotics Antimicrob Proteins. 2024. PMID: 38421575
-
Distribution, inducibility, and characteristics of Latilactobacillus curvatus temperate phages.Microbiome Res Rep. 2023 Sep 8;2(4):34. doi: 10.20517/mrr.2023.18. eCollection 2023. Microbiome Res Rep. 2023. PMID: 38045928 Free PMC article.
-
In-depth genome and pan-genome analysis of a metal-resistant bacterium Pseudomonas parafulva OS-1.Front Microbiol. 2023 Jun 20;14:1140249. doi: 10.3389/fmicb.2023.1140249. eCollection 2023. Front Microbiol. 2023. PMID: 37408640 Free PMC article.
-
Interplay between Inter-Subunit Rotation of the Ribosome and Binding of Translational GTPases.Int J Mol Sci. 2023 Apr 7;24(8):6878. doi: 10.3390/ijms24086878. Int J Mol Sci. 2023. PMID: 37108045 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
