Serial analysis of gene expression in the rat striatum following methamphetamine administration
- PMID: 17105900
- DOI: 10.1196/annals.1369.002
Serial analysis of gene expression in the rat striatum following methamphetamine administration
Abstract
Methamphetamine (METH), a highly addictive drug, can cause degeneration of monoaminergic terminals and neuronal apoptosis in the mammalian brain. In the present article, we have used serial analysis of gene expression (SAGE) to investigate patterns of gene expression in the striata of rats that were given a neurotoxic dose of the drug. SAGE libraries were generated from animals treated with either saline or METH (40 mg/kg) and sacrificed 2 h later. A total of 315 transcripts were differentially expressed between the two libraries (P < 0.05), with 179 (56%) being upregulated and 136 (44%) being downregulated by the METH injection. Of these, CAATT enhancer-binding protein homologous protein (CHOP)/GADD153 (growth arrest- and DNA damage-inducible gene 153) was found to be upregulated by about threefold. Analysis of the expression of genes downstream of CHOP (DOCs) revealed significant METH-induced increases in their expression. Because DOC1 is an analog of carbonic anhydrase (CA) which is involved in the interconversion between carbon dioxide and the bicarbonate ion, we also measured the effects of METH on the expression of several CAs. These were significantly upregulated by METH in a time-dependent fashion. These results indicate that METH toxicity is mediated, in part, by drug-induced perturbations of physiological processes that are dependent on normal pH and CO(2) homeostasis.
Similar articles
-
Dopamine quinones activate microglia and induce a neurotoxic gene expression profile: relationship to methamphetamine-induced nerve ending damage.Ann N Y Acad Sci. 2006 Aug;1074:31-41. doi: 10.1196/annals.1369.003. Ann N Y Acad Sci. 2006. PMID: 17105901
-
Profiling of methamphetamine-induced modifications of gene expression patterns in the mouse brain.Ann N Y Acad Sci. 2004 Oct;1025:76-83. doi: 10.1196/annals.1316.010. Ann N Y Acad Sci. 2004. PMID: 15542703
-
Methamphetamine-induced spectrin proteolysis in the rat striatum.J Neurochem. 2006 Mar;96(5):1267-76. doi: 10.1111/j.1471-4159.2005.03618.x. Epub 2006 Jan 17. J Neurochem. 2006. PMID: 16417574
-
Speed kills: cellular and molecular bases of methamphetamine-induced nerve terminal degeneration and neuronal apoptosis.FASEB J. 2003 Oct;17(13):1775-88. doi: 10.1096/fj.03-0073rev. FASEB J. 2003. PMID: 14519657 Review.
-
[Gene expression by addictive drugs and drug dependence].Nihon Shinkei Seishin Yakurigaku Zasshi. 2001 Feb;21(1):27-31. Nihon Shinkei Seishin Yakurigaku Zasshi. 2001. PMID: 11400323 Review. Japanese.
Cited by
-
Genetically correlated effects of selective breeding for high and low methamphetamine consumption.Genes Brain Behav. 2009 Nov;8(8):758-71. doi: 10.1111/j.1601-183X.2009.00522.x. Epub 2009 Jul 21. Genes Brain Behav. 2009. PMID: 19689456 Free PMC article.
-
Transcriptional response of rat frontal cortex following acute in vivo exposure to the pyrethroid insecticides permethrin and deltamethrin.BMC Genomics. 2008 Nov 18;9:546. doi: 10.1186/1471-2164-9-546. BMC Genomics. 2008. PMID: 19017407 Free PMC article.
-
Interrogating global gene expression in rat neuronal cultures using SAGE.Neurotox Res. 2007 Dec;12(4):209-14. doi: 10.1007/BF03033905. Neurotox Res. 2007. PMID: 18201949
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases
Research Materials