RE1 Silencing transcription factor maintains a repressive chromatin environment in embryonic hippocampal neural stem cells
- PMID: 17082226
- DOI: 10.1634/stemcells.2006-0207
RE1 Silencing transcription factor maintains a repressive chromatin environment in embryonic hippocampal neural stem cells
Abstract
The control of gene expression in neural stem cells is key to understanding their developmental and therapeutic potential, yet we know little of the transcriptional mechanisms that underlie their differentiation. Recent evidence has implicated the RE1 silencing transcription factor (REST) in neuronal differentiation. However, the means by which REST regulates transcription in neural stem cells remain unclear. Here, we show that REST recruits distinct corepressor platforms in neural stem cells. REST is able to both silence and repress neuronal genes in embryonic hippocampal neural stem cells by creating a chromatin environment that contains both repressive local epigenetic signature (characterized by low levels of histones H4 and H3K9 acetylation and elevated dimethylation of H3K9) and H3K4 methylation, which are characteristic of gene activation. Furthermore, inhibition of REST function leads to activation of several neuron-specific genes but does not lead to overt formation of mature neurons, supporting the notion that REST regulates part, but not all, of the neuronal differentiation program.
Similar articles
-
Small CTD phosphatases function in silencing neuronal gene expression.Science. 2005 Jan 28;307(5709):596-600. doi: 10.1126/science.1100801. Science. 2005. PMID: 15681389
-
Human positive coactivator 4 controls heterochromatinization and silencing of neural gene expression by interacting with REST/NRSF and CoREST.J Mol Biol. 2010 Mar 19;397(1):1-12. doi: 10.1016/j.jmb.2009.12.058. Epub 2010 Jan 18. J Mol Biol. 2010. PMID: 20080105
-
The repressor element 1-silencing transcription factor regulates heart-specific gene expression using multiple chromatin-modifying complexes.Mol Cell Biol. 2007 Jun;27(11):4082-92. doi: 10.1128/MCB.00269-07. Epub 2007 Mar 19. Mol Cell Biol. 2007. PMID: 17371849 Free PMC article.
-
Stemness is only a state of the cell.Cold Spring Harb Symp Quant Biol. 2008;73:227-34. doi: 10.1101/sqb.2008.73.042. Epub 2009 Jan 15. Cold Spring Harb Symp Quant Biol. 2008. PMID: 19150961 Review.
-
Transcriptional regulation: cancer, neurons and the REST.Curr Biol. 2005 Sep 6;15(17):R665-8. doi: 10.1016/j.cub.2005.08.032. Curr Biol. 2005. PMID: 16139198 Review.
Cited by
-
The Brain-Specific Neural Zinc Finger Transcription Factor 2b (NZF-2b/7ZFMyt1) Suppresses Cocaine Self-Administration in Rats.Front Behav Neurosci. 2010 Apr 5;4:14. doi: 10.3389/fnbeh.2010.00014. eCollection 2010. Front Behav Neurosci. 2010. PMID: 20407577 Free PMC article.
-
The rest repression of the neurosecretory phenotype is negatively modulated by BHC80, a protein of the BRAF/HDAC complex.J Neurosci. 2009 May 13;29(19):6296-307. doi: 10.1523/JNEUROSCI.5943-08.2009. J Neurosci. 2009. PMID: 19439607 Free PMC article.
-
REST corepressors RCOR1 and RCOR2 and the repressor INSM1 regulate the proliferation-differentiation balance in the developing brain.Proc Natl Acad Sci U S A. 2017 Jan 17;114(3):E406-E415. doi: 10.1073/pnas.1620230114. Epub 2017 Jan 3. Proc Natl Acad Sci U S A. 2017. PMID: 28049845 Free PMC article.
-
Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation.Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):E3535-44. doi: 10.1073/pnas.1504232112. Epub 2015 Jun 19. Proc Natl Acad Sci U S A. 2015. PMID: 26091879 Free PMC article.
-
The ATPase BRG1/SMARCA4 is a protein interaction platform that recruits BAF subunits and the transcriptional repressor REST/NRSF in neural progenitor cells.Mol Cell Biochem. 2019 Nov;461(1-2):171-182. doi: 10.1007/s11010-019-03600-0. Epub 2019 Aug 20. Mol Cell Biochem. 2019. PMID: 31428904
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
