doi: 10.1016/j.bbrc.2006.10.050.
Epub 2006 Oct 17.
Engineering of GAL1 promoter-driven expression system with artificial transcription factors
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- PMID: 17069762
- DOI: 10.1016/j.bbrc.2006.10.050
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Engineering of GAL1 promoter-driven expression system with artificial transcription factors
Biochem Biophys Res Commun.
.
Abstract
We isolated and characterized artificial transcription factors (ATFs) that functionally activate GAL1 promoter in yeast. These ATFs transformed the yeast galactose-dependent GAL1 promoter system into a galactose-independent one. The ATFs were identified by screening a combinatorial library of zinc finger-containing transcription factors for components that activated the transcription of a reporter gene under the control of a truncated GAL1 promoter from which the GAL4p-binding sites were deleted. We also showed that these ATFs activate transcription from GAL1 promoter by binding directly to specific sequence elements in the promoter.
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