Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Dec;7(12):1211-5.
doi: 10.1038/sj.embor.7400840. Epub 2006 Oct 27.

The cell nucleus taking centre stage. Workshop on the functional organization of the cell nucleus

Yosef Gruenbaum  1 , Ivan Raska, Harald Herrmann
Affiliations

The cell nucleus taking centre stage. Workshop on the functional organization of the cell nucleus

Yosef Gruenbaum et al. EMBO Rep. 2006 Dec.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Fluorescence microscopy of HeLa cells labelled for histone H3 Lys 27 trimethylation (H3K27me3) after forced expression of the lamin A-deletion mutant LAΔ50. (a–d) Control cells. (e–h) Cells expressing green fluorescent protein–lamin A (GFP–LA). (i–l) Cells expressing GFP–LAΔ50. GFP–LA-expressing cells displayed a normally shaped nucleus (e) and punctate H3K27me3 staining throughout the nucleus, with prominent arrays at the nuclear periphery (indicated by an asterisk; b,f), where it overlaps with the lamina (g,h). Expression of GFP–LAΔ50 caused lobe formations in the nuclear envelopes of most HeLa cells (i), and there was an overall loss of H3K27me3 staining, especially in the region of the lamina (j,k,l). Enlargements (×7.4) of the overlay areas indicated by white boxes (c,j,k) are shown in (d,h,l). Reproduced from Shumaker et al (2006) with the permission of the National Academy of Sciences.
Figure 2
Figure 2
Electron micrograph of a spread preparation of yeast nucleolar chromatin in the form of a Christmas tree. Many ribonucleoprotein structures appearing on nascent transcripts can now be characterized in functional terms. Scale bar, 0.5 mm. Reproduced from Osheim et al (2004) with the permission of Elsevier.
Figure 3
Figure 3
A schematic model of the supraspliceosome in which the pre-messenger RNA connects four native spliceosomes. Introns are shown in blue and exons are shown in red. The supraspliceosome presents a platform onto which the exons can be aligned and splice junctions can be checked before splicing occurs. Adapted from Azubel et al (2006).
None
Yosef Gruenbaum
None
Ivan Raska
None
Harald Herrmann
None
The third European Molecular Biology Organization (EMBO) workshop on the Functional Organization of the Cell Nucleus was held in Prague between 5 and 8 May 2006, and was organized by U. Aebi, I. Raska and W. Earnshaw.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Azubel M, Habib N, Sperling R, Sperling J (2006) Native spliceosomes assemble with pre-mRNA to form supraspliceosomes. J Mol Biol 356: 955–966 - PubMed
    1. Blow JJ, Dutta A (2005) Preventing re-replication of chromosomal DNA. Nat Rev Cell Mol Biol 6: 476–486 - PMC - PubMed
    1. Conti E, Muller CW, Stewart M (2006) Karyopherin flexibility in nucleocytoplasmic transport. Curr Opin Struct Biol 16: 237–244 - PubMed
    1. Cremer T, Cremer C (2001) Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat Rev Genet 2: 292–301 - PubMed
    1. Grimaud C, Negre N, Cavalli G (2006) From genetics to epigenetics: the tale of Polycomb group and trithorax group genes. Chromosome Res 14: 363–375 - PubMed