Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer
- PMID: 17013394
- DOI: 10.1038/ng1895
Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer
Abstract
Since the creation of Dolly via somatic cell nuclear transfer (SCNT), more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1%-5%) is that the clones are likely to be derived from adult stem cells. Support for this hypothesis comes from the findings that the reproductive cloning efficiency for embryonic stem cells is five to ten times higher than that for somatic cells as donors and that cloned pups cannot be produced directly from cloned embryos derived from differentiated B and T cells or neuronal cells. The question remains as to whether SCNT-derived animal clones can be derived from truly differentiated somatic cells. We tested this hypothesis with mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells, progenitor cells and granulocytes. We found that cloning efficiency increases over the differentiation hierarchy, and terminally differentiated postmitotic granulocytes yield cloned pups with the greatest cloning efficiency.
Comment in
-
On the cloning of animals from terminally differentiated cells.Nat Genet. 2007 Feb;39(2):136-7; author reply 137-8. doi: 10.1038/ng0207-136. Nat Genet. 2007. PMID: 17262020 No abstract available.
Similar articles
-
Recloned dogs derived from adipose stem cells of a transgenic cloned beagle.Theriogenology. 2011 Apr 15;75(7):1221-31. doi: 10.1016/j.theriogenology.2010.11.035. Epub 2011 Jan 8. Theriogenology. 2011. PMID: 21220163 Clinical Trial.
-
Cloning mice and ES cells by nuclear transfer from somatic stem cells and fully differentiated cells.Methods Mol Biol. 2011;770:551-69. doi: 10.1007/978-1-61779-210-6_22. Methods Mol Biol. 2011. PMID: 21805280
-
Monoclonal mice generated by nuclear transfer from mature B and T donor cells.Nature. 2002 Feb 28;415(6875):1035-8. doi: 10.1038/nature718. Epub 2002 Feb 10. Nature. 2002. PMID: 11875572
-
Nuclear cloning, epigenetic reprogramming and cellular differentiation.Novartis Found Symp. 2005;265:107-18; discussion 118-28. Novartis Found Symp. 2005. PMID: 16050253 Review.
-
Production of cloned mice and ES cells from adult somatic cells by nuclear transfer: how to improve cloning efficiency?J Reprod Dev. 2007 Feb;53(1):13-26. doi: 10.1262/jrd.18120. J Reprod Dev. 2007. PMID: 17332696 Review.
Cited by
-
Dual function of histone H3 lysine 36 methyltransferase ASH1 in regulation of Hox gene expression.PLoS One. 2011;6(11):e28171. doi: 10.1371/journal.pone.0028171. Epub 2011 Nov 28. PLoS One. 2011. PMID: 22140534 Free PMC article.
-
An opposite effect of the CDK inhibitor, p18(INK4c) on embryonic stem cells compared with tumor and adult stem cells.PLoS One. 2012;7(9):e45212. doi: 10.1371/journal.pone.0045212. Epub 2012 Sep 26. PLoS One. 2012. PMID: 23049777 Free PMC article.
-
Proteome of mouse oocytes at different developmental stages.Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17639-44. doi: 10.1073/pnas.1013185107. Epub 2010 Sep 27. Proc Natl Acad Sci U S A. 2010. PMID: 20876089 Free PMC article.
-
Somatic donor cell type correlates with embryonic, but not extra-embryonic, gene expression in postimplantation cloned embryos.PLoS One. 2013 Oct 16;8(10):e76422. doi: 10.1371/journal.pone.0076422. eCollection 2013. PLoS One. 2013. PMID: 24146866 Free PMC article.
-
Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate non-integrated iPSC reprogramming of human myeloid progenitors.PLoS One. 2012;7(8):e42838. doi: 10.1371/journal.pone.0042838. Epub 2012 Aug 8. PLoS One. 2012. PMID: 22905176 Free PMC article.
Publication types
MeSH terms
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases
