Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Oct;1(10):1103-11.
doi: 10.1002/biot.200600143.

Use of virus vectors for the expression in plants of active full-length and single chain anti-coronavirus antibodies

Josefa M Alamillo  1 , Wendy Monger, Isabel Sola, Beatriz García, Yolande Perrin, Marco Bestagno, Oscar R Burrone, Patricia Sabella, Joan Plana-Durán, Luis Enjuanes, George P Lomonossoff, Juan A García
Affiliations

Use of virus vectors for the expression in plants of active full-length and single chain anti-coronavirus antibodies

Josefa M Alamillo et al. Biotechnol J. 2006 Oct.

Abstract

To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full-length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The alphaSIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the alpha-CH3 domain from human IgA. To express the full-length IgA, the individual light and heavy chains from the TGEV-specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co-infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant-expressed alphaSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody-expressing plant tissue to 2-day-old piglets showed that both the alphaSIP and full-length IgA molecules can provide in vivo protection against TGEV.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Enjuanes, L., van der Zeijst, B. A. M., Molecular basis of TGE coronavirus epidemiology. In: Siddell, S. G. (Ed.), The Coronaviridae, Plenum Press, New York 1995, pp. 337‐376.
    1. Gebauer, F. , Posthumus, W. A. P. , Correa, I. , Suñé, C. et al., Residues involved in the formation of the antigenic sites of the S protein of transmissible gastroenteritis coronavirus. Virology 1991. 183, 225–238. - PMC - PubMed
    1. Castilla, J. , Sola, I. , Enjuanes, L. , Interference of coronavirus infection by expression of immunoglobulin G (IgG) or IgA virus‐neutralizing antibodies. J. Virol. 1997. 71, 5251–5258. - PMC - PubMed
    1. Sola, I. , Castilla, J. , Pintado, B. , Sánchez‐Morgado, J. M. et al., Transgenic mice secreting coronavirus neutralizing antibodies into the milk. J. Virol. 1998. 72, 3762–3771. - PMC - PubMed
    1. Li, E. , Pedraza, A. , Bestagno, M. , Mancardi, S. et al., Mammalian cell expression of dimeric small immune proteins (SIP). Protein Eng. 1997. 10, 731–736. - PubMed

Publication types

MeSH terms

LinkOut - more resources