Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Mar;7(1):37-50.
doi: 10.1007/s10969-006-9008-x. Epub 2006 Jul 12.

Crystal structure of the vitamin B12 biosynthetic cobaltochelatase, CbiXS, from Archaeoglobus fulgidus

Jiang Yin  1 , Linda X Xu, Maia M Cherney, Evelyne Raux-Deery, Amanda A Bindley, Alexei Savchenko, John R Walker, Marianne E Cuff, Martin J Warren, Michael N G James
Affiliations

Crystal structure of the vitamin B12 biosynthetic cobaltochelatase, CbiXS, from Archaeoglobus fulgidus

Jiang Yin et al. J Struct Funct Genomics. 2006 Mar.

Abstract

The Archaeoglobus fulgidus gene af0721 encodes CbiX(S), a small cobaltochelatase associated with the anaerobic biosynthesis of vitamin B12 (cobalamin). The protein was shown to have activity both in vivo and in vitro, catalyzing the insertion of Co2+ into sirohydrochlorin. The structure of CbiX(S) was determined in two different crystal forms and was shown to consist of a central mixed beta-sheet flanked by four alpha-helices, one of which originates in the C-terminus of a neighboring molecule. CbiX(S) is about half the size of other Class II tetrapyrrole chelatases. The overall topography of CbiX(S) exhibits substantial resemblance to both the N- and C-terminal regions of several members of the Class II metal chelatases involved in tetrapyrrole biosynthesis. Two histidines (His10 and His74), are in similar positions as the catalytic histidine residues in the anaerobic cobaltochelatase CbiK (His145 and His207). In light of the hypothesis that suggests the larger chelatases evolved via gene duplication and fusion from a CbiX(S)-like enzyme, the structure of AF0721 may represent that of an "ancestral" precursor of class II metal chelatases.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Structure. 1997 Nov 15;5(11):1501-10 - PubMed
    1. Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):849-61 - PubMed
    1. Biochem J. 1997 Oct 15;327 ( Pt 2):321-33 - PubMed
    1. J Mol Biol. 1993 Jan 5;229(1):105-24 - PubMed
    1. J Mol Biol. 2000 Mar 17;297(1):221-32 - PubMed

Publication types

MeSH terms

LinkOut - more resources