doi: 10.1038/nm1437.
Epub 2006 Jun 25.
Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing
Affiliations
- PMID: 16799556
- DOI: 10.1038/nm1437
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Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing
Nat Med.
2006 Jul.
Erratum in
- Nat Med. 2006 Oct;12(10):1220
Abstract
The sensitivity of conventional DNA sequencing in tumor biopsies is limited by stromal contamination and by genetic heterogeneity within the cancer. Here, we show that microreactor-based pyrosequencing can detect rare cancer-associated sequence variations by independent and parallel sampling of multiple representatives of a given DNA fragment. This technology can thereby facilitate accurate molecular diagnosis of heterogeneous cancer specimens and enable patient selection for targeted cancer therapies.
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