The effect of phosphatidylserine on in vitro hydroxyapatite growth and proliferation
- PMID: 1657326
- DOI: 10.1007/BF02556117
The effect of phosphatidylserine on in vitro hydroxyapatite growth and proliferation
Abstract
The acidic phospholipid phosphatidylserine (PS) has been reported to have variable effects on in vitro hydroxyapatite proliferation. PS promotes in vitro mineralization in systems in which calcium-PS-phosphate complexes are allowed to form, and inhibits in vitro mineralization when incorporated into liposomes. To investigate these diverse effects, a Langmuir adsorption isotherm was used to determine the affinity of PS for hydroxyapatite crystals, based on binding of 14C-PS to synthetic hydroxyapatite crystals of specific surface 54 m2/g. Using this model, PS was found to bind to hydroxyapatite crystals with an affinity comparable to that of the amino acid phosphoserine (K = 3.33 ml/mumol). Coating the surface of hydroxyapatite seed crystals with PS reduced their rate of proliferation in a metastable calcium phosphate solution in which calcium-PS-phosphate complexes were previously shown to promote hydroxyapatite formation. The extent of inhibition of hydroxyapatite seeded growth was directly related to the proportion of the hydroxyapatite surface covered with PS. These data suggest that PS may have multiple effects on hydroxyapatite formation in situ, and that mineral-PS interactions can retard crystal proliferation.
Similar articles
-
Persistence of complexed acidic phospholipids in rapidly mineralizing tissues is due to affinity for mineral and resistance to hydrolytic attack: in vitro data.Calcif Tissue Int. 1996 Jan;58(1):45-51. doi: 10.1007/BF02509545. Calcif Tissue Int. 1996. PMID: 8825238
-
Hyaluronan interactions with hydroxyapatite do not alter in vitro hydroxyapatite crystal proliferation and growth.Matrix. 1991 Dec;11(6):442-6. doi: 10.1016/s0934-8832(11)80198-8. Matrix. 1991. PMID: 1664910
-
The role of synthetic and bone extracted Ca-phospholipid-PO4 complexes in hydroxyapatite formation.Calcif Tissue Res. 1977 Oct 20;23(3):251-8. doi: 10.1007/BF02012794. Calcif Tissue Res. 1977. PMID: 902143
-
Mineral-matrix interactions in bone and cartilage.Clin Orthop Relat Res. 1992 Aug;(281):244-74. Clin Orthop Relat Res. 1992. PMID: 1323440 Review.
-
Osteocalcin-hydroxyapatite interaction in the extracellular organic matrix of bone.Anat Rec. 1989 Jun;224(2):180-8. doi: 10.1002/ar.1092240208. Anat Rec. 1989. PMID: 2549810 Review.
Cited by
-
Human proximal tubular cells can form calcium phosphate deposits in osteogenic culture: role of cell death and osteoblast-like transdifferentiation.Cell Death Discov. 2019 Jan 28;5:57. doi: 10.1038/s41420-019-0138-x. eCollection 2019. Cell Death Discov. 2019. PMID: 30701089 Free PMC article.
-
Persistence of complexed acidic phospholipids in rapidly mineralizing tissues is due to affinity for mineral and resistance to hydrolytic attack: in vitro data.Calcif Tissue Int. 1996 Jan;58(1):45-51. doi: 10.1007/BF02509545. Calcif Tissue Int. 1996. PMID: 8825238
-
Phosphoserine--a convenient compound for modification of calcium phosphate bone cement collagen composites.J Mater Sci Mater Med. 2004 Apr;15(4):451-5. doi: 10.1023/b:jmsm.0000021119.14870.3d. J Mater Sci Mater Med. 2004. PMID: 15332616
-
Suramin-induced mucopolysaccharidosis in rat incisor.Cell Tissue Res. 1993 Jul;273(1):53-64. doi: 10.1007/BF00304611. Cell Tissue Res. 1993. PMID: 8364961
-
The nucleation and growth of calcium phosphate by amelogenin.J Cryst Growth. 2007 Jun 15;304(2):407-415. doi: 10.1016/j.jcrysgro.2007.02.035. J Cryst Growth. 2007. PMID: 19079557 Free PMC article.