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. 1991 Oct;65(10):5647-52.
doi: 10.1128/JVI.65.10.5647-5652.1991.

The T/t common region of simian virus 40 large T antigen contains a distinct transformation-governing sequence

E Marsilio  1 , S H Cheng, B Schaffhausen, E Paucha, D M Livingston
Affiliations

The T/t common region of simian virus 40 large T antigen contains a distinct transformation-governing sequence

E Marsilio et al. J Virol. 1991 Oct.

Abstract

Simian virus 40 large T antigen (T) can transform cultured cells, but the mechanisms by which it functions are not entirely understood. Several lines of evidence have suggested that the amino-terminal approximately 130 residues of T may be sufficient to confer the transforming capability. Oligonucleotide-directed mutagenesis was used to generate a series of deletion and substitution mutants within the amino-terminal 82 residues of T, the segment which is shared with simian virus 40 small t antigen (t). Results of stability and transformation assays of these mutants strongly suggest that the 1-to-82 region of T contains sequences which govern T transforming activity and affect in vivo stability. Instability and a defect in transforming activity could be separated from one another genetically. Thus, the 1-to-82 region appears to contain a specific region that contributes to the transforming function of the protein. This segment operates by means other than the simple binding of pRb and/or p107.

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