Adiponectin stimulates proliferation and cytokine secretion in colonic epithelial cells
- PMID: 16529829
- DOI: 10.1016/j.regpep.2006.02.001
Adiponectin stimulates proliferation and cytokine secretion in colonic epithelial cells
Abstract
Adiponectin is a recently described mediator secreted by adipose tissue. Here we report the growth promoting and pro-inflammatory actions of adiponectin on colonic epithelial cancer cells. Full-length and globular adiponectin produced an identical stimulation of HT-29 cell growth that was blocked by inhibition of adenylate cyclase and protein kinase A and partially inhibited by a pan-specific protein kinase C inhibitor, but was unaffected by specific inhibition of extracellular signal-related kinase (ERK) or p38 MAP kinase. Globular adiponectin but not full-length adiponectin significantly increased the secretion and mRNA levels of IL-8, GM-CSF and MCP-1. Globular adiponectin doubled IL-1beta-stimulated IL-8 and GM-CSF secretion. Adiponectin-stimulated cytokine secretion was blocked by pharmacological inhibitors of NF-kappaB, ERK and p38 MAP kinase. Globular adiponectin increased phosphorylation of both ERK and p38 MAP kinase and increased the nuclear translocation of active NF-kappaB. Adiponectin has pro-proliferative and pro-inflammatory actions on colonic epithelial cells; these appear to be differentially activated by the adiponectin isoforms. Adiponectin may have a role in the regulation of gastrointestinal mucosal function, inflammation and colon carcinogenesis.
Similar articles
-
Tumor necrosis factor-alpha regulates inflammatory and mesenchymal responses via mitogen-activated protein kinase kinase, p38, and nuclear factor kappaB in human endometriotic epithelial cells.Mol Pharmacol. 2008 May;73(5):1394-404. doi: 10.1124/mol.107.042176. Epub 2008 Feb 5. Mol Pharmacol. 2008. PMID: 18252806
-
Mitogen-activated protein kinase modulation of nuclear factor-kappaB-induced granulocyte macrophage-colony-stimulating factor release from human alveolar macrophages.Am J Respir Cell Mol Biol. 2004 Mar;30(3):342-9. doi: 10.1165/rcmb.2003-0122OC. Epub 2003 Jul 18. Am J Respir Cell Mol Biol. 2004. PMID: 12871851
-
Globular adiponectin induces a pro-inflammatory response in human astrocytic cells.Biochem Biophys Res Commun. 2014 Mar 28;446(1):37-42. doi: 10.1016/j.bbrc.2014.02.077. Epub 2014 Feb 25. Biochem Biophys Res Commun. 2014. PMID: 24582565
-
p38 MAP kinase and MKK-1 co-operate in the generation of GM-CSF from LPS-stimulated human monocytes by an NF-kappa B-independent mechanism.Br J Pharmacol. 2000 Nov;131(6):1143-53. doi: 10.1038/sj.bjp.0703684. Br J Pharmacol. 2000. PMID: 11082122 Free PMC article.
-
Inhibitors of mitogen-activated protein kinases differentially regulate eosinophil-activating cytokine release from human airway smooth muscle.Am J Respir Crit Care Med. 2001 Aug 15;164(4):688-97. doi: 10.1164/ajrccm.164.4.2011004. Am J Respir Crit Care Med. 2001. PMID: 11520738
Cited by
-
Effect of exercise on chemically-induced colitis in adiponectin deficient mice.J Inflamm (Lond). 2012 Aug 21;9(1):30. doi: 10.1186/1476-9255-9-30. J Inflamm (Lond). 2012. PMID: 22909126 Free PMC article.
-
Impact of adiponectin deficiency on pulmonary responses to acute ozone exposure in mice.Am J Respir Cell Mol Biol. 2010 Oct;43(4):487-97. doi: 10.1165/rcmb.2009-0086OC. Epub 2009 Nov 13. Am J Respir Cell Mol Biol. 2010. PMID: 19915153 Free PMC article.
-
Leptin and Adiponectin: new players in the field of tumor cell and leukocyte migration.Cell Commun Signal. 2009 Dec 23;7:27. doi: 10.1186/1478-811X-7-27. Cell Commun Signal. 2009. PMID: 20030801 Free PMC article.
-
The role of obesity in oesophageal cancer development.Therap Adv Gastroenterol. 2014 Nov;7(6):247-68. doi: 10.1177/1756283X14538689. Therap Adv Gastroenterol. 2014. PMID: 25364384 Free PMC article. Review.
-
Involvement of adiponectin in early stage of colorectal carcinogenesis.BMC Cancer. 2014 Nov 5;14:811. doi: 10.1186/1471-2407-14-811. BMC Cancer. 2014. PMID: 25370174 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
