Chromatin remodeling by ISW2 and SWI/SNF requires DNA translocation inside the nucleosome
- PMID: 16518397
- DOI: 10.1038/nsmb1071
Chromatin remodeling by ISW2 and SWI/SNF requires DNA translocation inside the nucleosome
Abstract
Chromatin-remodeling complexes regulate access to nucleosomal DNA by mobilizing nucleosomes in an ATP-dependent manner. In this study, we find that chromatin remodeling by SWI/SNF and ISW2 involves DNA translocation inside nucleosomes two helical turns from the dyad axis at superhelical location-2. DNA translocation at this internal position does not require the propagation of a DNA twist from the site of translocation to the entry/exit sites for nucleosome movement. Nucleosomes are moved in 9- to 11- or approximately 50-base-pair increments by ISW2 or SWI/SNF, respectively, presumably through the formation of DNA loops on the nucleosome surface. Remodeling by ISW2 but not SWI/SNF requires DNA torsional strain near the site of translocation, which may work in conjunction with conformational changes of ISW2 to promote nucleosome movement on DNA. The difference in step size of nucleosome movement by SWI/SNF and ISW2 demonstrates how SWI/SNF may be more disruptive to nucleosome structure than ISW2.
Similar articles
-
Rad4-Rad23 interaction with SWI/SNF links ATP-dependent chromatin remodeling with nucleotide excision repair.Nat Struct Mol Biol. 2006 Oct;13(10):902-7. doi: 10.1038/nsmb1152. Epub 2006 Oct 1. Nat Struct Mol Biol. 2006. PMID: 17013386
-
Nucleosome mobilization catalysed by the yeast SWI/SNF complex.Nature. 1999 Aug 19;400(6746):784-7. doi: 10.1038/23506. Nature. 1999. PMID: 10466730
-
Functional role of extranucleosomal DNA and the entry site of the nucleosome in chromatin remodeling by ISW2.Mol Cell Biol. 2004 Nov;24(22):10047-57. doi: 10.1128/MCB.24.22.10047-10057.2004. Mol Cell Biol. 2004. PMID: 15509805 Free PMC article.
-
Remodeling chromatin structures for transcription: what happens to the histones?Bioessays. 1996 Nov;18(11):875-84. doi: 10.1002/bies.950181106. Bioessays. 1996. PMID: 8939065 Review.
-
Nucleosome organization and targeting of SWI/SNF chromatin-remodeling complexes: contributions of the DNA sequence.Biochem Cell Biol. 2007 Aug;85(4):419-25. doi: 10.1139/O07-070. Biochem Cell Biol. 2007. PMID: 17713577 Review.
Cited by
-
RSC primes the quiescent genome for hypertranscription upon cell-cycle re-entry.Elife. 2021 May 27;10:e67033. doi: 10.7554/eLife.67033. Elife. 2021. PMID: 34042048 Free PMC article.
-
Structural basis for recognition and remodeling of the TBP:DNA:NC2 complex by Mot1.Elife. 2015 Aug 10;4:e07432. doi: 10.7554/eLife.07432. Elife. 2015. PMID: 26258880 Free PMC article.
-
Regulation of ISW2 by concerted action of histone H4 tail and extranucleosomal DNA.Mol Cell Biol. 2006 Oct;26(20):7388-96. doi: 10.1128/MCB.01159-06. Mol Cell Biol. 2006. PMID: 17015471 Free PMC article.
-
Mechanisms of ATP dependent chromatin remodeling.Mutat Res. 2007 May 1;618(1-2):3-17. doi: 10.1016/j.mrfmmm.2006.08.015. Epub 2007 Jan 21. Mutat Res. 2007. PMID: 17306844 Free PMC article. Review.
-
Histone H1 subtypes differentially modulate chromatin condensation without preventing ATP-dependent remodeling by SWI/SNF or NURF.PLoS One. 2009 Oct 1;4(10):e0007243. doi: 10.1371/journal.pone.0007243. PLoS One. 2009. PMID: 19794910 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
